Centre for Cellular and Molecular Biology (CCMB), Council for Scientific and Industrial Research (CSIR), Hyderabad, India.
J Sep Sci. 2011 Jan;34(2):225-32. doi: 10.1002/jssc.201000626. Epub 2010 Dec 2.
Zebrafish (Danio rerio) is the widely used vertebrate model animal for understanding the complexity of development and disease process. Zebrafish has been also extensively used in understanding the mechanism of regeneration for its extensive capability of regenerating fins and other tissues. We have analyzed the proteome profile of zebrafish caudal fin in its native state based on one-dimensional gel electrophoresis LCMS/MS and two-dimensional gel electrophoresis MS/MS analyses. A total of 417 proteins were identified as zebrafish fin tissue specific, which includes 397 proteins identified based on one-dimensional gel electrophoresis LCMS/MS analysis and 101 proteins identified based on two-dimensional gel electrophoresis MALDI MS/MS. The proteins mapped to the zebrafish fin tissue were shown to be involved in various biological activities related to development, apoptosis, signaling and metabolic process. Focal adhesion, regulation of actin cytoskeleton, cancer-related pathways, mitogen-activated protein kinase signaling, antigen processing and presentation, and proteasome are some of the important pathways associated with the identified proteome data set of the zebrafish fin.
斑马鱼（Danio rerio）是一种广泛应用于理解发育和疾病过程复杂性的脊椎动物模式生物。斑马鱼还广泛用于理解再生机制，因为它具有很强的再生鳍和其他组织的能力。我们已经基于一维凝胶电泳 LCMS/MS 和二维凝胶电泳 MS/MS 分析，分析了斑马鱼尾鳍的蛋白质组图谱。总共鉴定出 417 种蛋白质是斑马鱼鳍组织特异性的，其中 397 种蛋白质是基于一维凝胶电泳 LCMS/MS 分析鉴定的，101 种蛋白质是基于二维凝胶电泳 MALDI MS/MS 鉴定的。映射到斑马鱼鳍组织的蛋白质被证明参与了与发育、细胞凋亡、信号转导和代谢过程相关的各种生物学活动。黏着斑、肌动蛋白细胞骨架调节、癌症相关途径、有丝分裂原激活蛋白激酶信号转导、抗原加工和呈递以及蛋白酶体是与斑马鱼鳍鉴定蛋白质组数据集相关的一些重要途径。
