Department of Neurobiology, CCMB, Hyderabad, India.
Zebrafish. 2010 Jun;7(2):169-77. doi: 10.1089/zeb.2010.0657.
Zebrafish is one of the most widely studied model organisms for understanding the neurodevelopment and neurological disorders of humans because of its similar brain structure, genome, and proteome. Understanding the zebrafish brain proteome is particularly useful as no data of zebrafish brain proteome are available despite its wide use as an alternative neurological animal model. We have determined the proteome profile of the zebrafish brain based on two-dimensional gel electrophoresis covering 161 proteins including 96 protein identities reported to the Swiss-Prot database. The proteins identified in this study were found to be associated with various pathways such as cell death, free radical scavenging, cell signaling, nervous system development, and cell cycle. The identified proteins were also observed to play important roles in various brain-related diseases and genetic disorders, such as Huntington's disease, Parkinson's disease, and schizophrenia. This article provides the zebrafish brain two-dimensional gel electrophoresis proteome map and the details of the 161 brain tissue-specific proteins. Also we have established the roles of the identified proteins in various neurological functions and diseases based on pathway analysis.
斑马鱼由于其相似的脑结构、基因组和蛋白质组，成为研究人类神经发育和神经紊乱的最广泛的模式生物之一。了解斑马鱼的脑蛋白质组特别有用，尽管它被广泛用作替代神经动物模型，但目前尚无斑马鱼脑蛋白质组的数据。我们已经根据二维凝胶电泳确定了斑马鱼脑的蛋白质组图谱，该图谱涵盖了包括 96 种蛋白质身份的 161 种蛋白质，这些蛋白质已被报道到瑞士 - 蛋白数据库。在这项研究中鉴定的蛋白质被发现与各种途径有关，如细胞死亡、自由基清除、细胞信号转导、神经系统发育和细胞周期。鉴定的蛋白质还被观察到在各种与大脑相关的疾病和遗传疾病中发挥重要作用，如亨廷顿病、帕金森病和精神分裂症。本文提供了斑马鱼脑二维凝胶电泳蛋白质组图谱和 161 种脑组织特异性蛋白质的详细信息。我们还根据途径分析确定了所鉴定蛋白质在各种神经功能和疾病中的作用。
