通过核磁共振和核酸酶图谱分析对酵母苯丙氨酸转运核糖核酸前体的体外转录本进行结构研究。

Hall K B, Sampson J R

Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St. Louis, MO 63110.

Nucleic Acids Res. 1990 Dec 11;18(23):7041-7. doi: 10.1093/nar/18.23.7041.

Both NMR and nuclease mapping have been used to probe the structure of an unmodified yeast tRNA(phe) precursor synthesized in vitro by T7 RNA polymerase. A comparison of the NMR data of the precursor and of the mature tRNA transcript shows that the mature tRNA domain structure is similar in both molecules. In the tRNA precursor, the intron consists of a stem of at least four base-pairs, identified by NMR, and two single-stranded loops, identified by nuclease mapping. This is in agreement with the structure previously proposed for the native tRNA(phe) precursor (1). However, our data also show the intron structure to be less stable than the mature tRNA domain, suggesting that the precursor may best be described as having two domains with a hinge at the junction of the anticodon and intron stems.

核磁共振（NMR）和核酸酶图谱分析都已用于探测由T7 RNA聚合酶体外合成的未修饰酵母tRNA（苯丙氨酸）前体的结构。对前体和成熟tRNA转录本的NMR数据进行比较表明，两种分子中成熟tRNA的结构域结构相似。在tRNA前体中，内含子由至少四个碱基对的茎（通过NMR鉴定）和两个单链环（通过核酸酶图谱分析鉴定）组成。这与先前针对天然tRNA（苯丙氨酸）前体提出的结构一致（1）。然而，我们的数据还表明内含子结构不如成熟tRNA结构域稳定，这表明前体最好被描述为具有两个结构域，在反密码子和内含子茎的连接处有一个铰链。