Ogden R C, Lee M C, Knapp G
Nucleic Acids Res. 1984 Dec 21;12(24):9367-82. doi: 10.1093/nar/12.24.9367.
The primary sequences of all the tRNA precursors which contain intervening sequences and which accumulate in the Saccharomyces cerevisiae rnal mutant are presented. A combination of DNA and RNA sequence analysis has led to elucidation of the primary sequence of four hitherto uncharacterized precursors. The location of the intervening sequence has in all cases been unambiguously determined by analysis of the intermediates in the splicing reaction. Secondary structures based upon the tRNA cloverleaf are shown for all the tRNA precursors and discussed with respect to common recognition by the yeast splicing endonuclease.
本文列出了所有含有居间序列并在酿酒酵母rna突变体中积累的trna前体的一级序列。DNA和RNA序列分析相结合，阐明了四种迄今未被表征的前体的一级序列。通过对剪接反应中间体的分析，在所有情况下都明确确定了居间序列的位置。展示了所有trna前体基于trna三叶草结构的二级结构，并就酵母剪接内切核酸酶的共同识别进行了讨论。
