Chen Yan, Lu Hui, Song Shihua, Jia Xiaobin
Key Laboratory of New Drug Delivery System of Chinese Meteria Medica, Key Laboratory of Scientific Preparation for Clinical Effective Prescription of State Administration of Traditional Chinese Medicine, Jiangsu Provincial Academy of Chinese Medicine, Nanjing 210028, China.
Zhongguo Zhong Yao Za Zhi. 2010 Oct;35(20):2679-83.
To research the microemulsion preparation of Ganoderma lucidum polysaccharides and triterpenes and investigate its properities. Evaluate the effects of polysaccharides and triterpenes microemulsions against transplant tumor growth.
The microemulsion formula was optimized by constructing the pseudo-ternary phase diagrams of blank microemulsion. The polysaccharides and triterpenes microemulsions were prepared on the blank microemulsions. The appearance, particle distribution and Zeta potential were investigated by transmission electron microscope and grain size analyzer. The Heps mice were randomly administered with polysaccharides and triterpenes microemulsions (114.5, 57.25 mg x kg(-1) x d(-1)) for 7 days. The effectiveness was assessed based on tumor inhibitory ratio of mice with Heps tumors. The toxicity was evaluated by measurements of the mice weight, immune organ weight.
The optimal microemulsion formula was composed of tween 20, dimethyl carbinol, water and 9-octadecenoic acid with the ratio of 14.3: 14.3: 33. 3:2. Polysaccharides and triterpenes microemulsions in transmission electron microscope were consisted of small spherical drop. The average particle size was 32.43 nm and the Zeta potential was -3.41 mV. The polysaccharides and triterpenes microemulsions showed an inhibition rate of 37.66% (57.25 mg x kg(-1) x d(-1)) and 52.34% (114.5 mg x kg(-1) x d(-1)) respectively against Heps tumor growth.
The acquired microemulsion with small particle size is stable. It significantly inhibits the tumor growth in Heps mice.
研究灵芝多糖和三萜的微乳制剂并考察其性质。评价多糖和三萜微乳对移植瘤生长的影响。
通过构建空白微乳的伪三元相图优化微乳配方。在空白微乳基础上制备多糖和三萜微乳。采用透射电子显微镜和粒度分析仪考察其外观、粒径分布及Zeta电位。将Heps小鼠随机给予多糖和三萜微乳（114.5、57.25mg·kg⁻¹·d⁻¹），连续给药7天。根据Heps荷瘤小鼠的肿瘤抑制率评估有效性。通过测量小鼠体重、免疫器官重量评价毒性。
最佳微乳配方由吐温20、异丙醇、水和油酸组成，比例为14.3∶14.3∶33.3∶2。透射电子显微镜下多糖和三萜微乳呈小球形液滴。平均粒径为32.43nm，Zeta电位为-3.41mV。多糖和三萜微乳对Heps肿瘤生长的抑制率分别为37.66%（57.25mg·kg⁻¹·d⁻¹）和52.34%（114.5mg·kg⁻¹·d⁻¹）。
所制备的微乳粒径小且稳定。对Heps小鼠肿瘤生长有显著抑制作用。
