Institute of Pharmaceutics, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, PR China.
J Pharm Pharmacol. 2010 Jan;62(1):139-44. doi: 10.1211/jpp.62.01.0016.
The aim was to prepare novel Ganoderma lucidum polysaccharide nanoparticles and to evaluate the physicochemical properties and anti-tumour activity in in-vitro cytotoxicity studies using HepG2, HeLa and A549 cancer cell lines, and growth promotion effects on mouse spleen cells.
Chitosan nanoparticles loaded with G. lucidum polysaccharide were prepared using the ion-revulsion method. The diameter distribution of the particles and the surface charge were measured using a zetasizer analyser. The entrapment efficiency and drug loading capacity were examined by the diethylaminoethanol weak anion exchange method. The cytotoxic effects of nanoparticles on tumour cells and the growth promotion effects on mouse spleen cells were tested using the MTT assay.
Nanoparticles loaded with G. lucidum polysaccharide at 6 microg/ml and chitosan/sodium tripolyphosphate (mass) ratio of 5.5 had significantly greater cytotoxic effects on tumour cells and growth promotion effects on mouse spleen cells than empty nanoparticles.
G. lucidum polysaccharide nanoparticles showed significant anti-tumour efficacy, having both cytotoxic effects on tumour cells and growth promotion effects on spleen cells, making it a promising candidate in the clinical setting.
制备新型灵芝多糖纳米粒，并通过 HepG2、HeLa 和 A549 癌细胞系的体外细胞毒性研究评估其理化性质和抗肿瘤活性，以及对小鼠脾细胞的生长促进作用。
采用离子排斥法制备壳聚糖纳米粒负载灵芝多糖。使用纳米粒度分析仪测量颗粒的粒径分布和表面电荷。通过二乙氨基乙醇弱阴离子交换法测定包封效率和载药量。使用 MTT 法测定纳米粒对肿瘤细胞的细胞毒性作用和对小鼠脾细胞的生长促进作用。
负载灵芝多糖的纳米粒（浓度为 6 μg/ml，壳聚糖/三聚磷酸钠（质量）比为 5.5）对肿瘤细胞的细胞毒性作用和对小鼠脾细胞的生长促进作用明显大于空白纳米粒。
灵芝多糖纳米粒具有显著的抗肿瘤疗效，对肿瘤细胞具有细胞毒性作用，对脾细胞具有生长促进作用，在临床应用中具有广阔的应用前景。
