利什曼原虫主要亚种的同工酶电泳特征分析，利什曼原虫主要亚种是沙特阿拉伯北部和西部人畜共患皮肤利什曼病的病原体。
Isoenzyme electrophetic characterization of Leishmania major, the causative agent of zoonotic cutaneous Leishmaniasis in North and West Saudi Arabia.
作者信息
Bin Dajem Saad M, Mostafa Osama M S, Abdoon Abdullmohsen, Al-Quraishy Saleh A S, Alqahtani Ahmed A
机构信息
Department of Biology, Faculty of Science, King Khalid University, Abha, Saudi Arabia.
出版信息
J Egypt Soc Parasitol. 2010 Aug;40(2):465-78.
Several expeditions were carried out to four localities (Al-Madinah Almona-warah, Tabouk region, Al-Jouf and Northern Frontiers regions) in Northern and Western Saudi Arabia for sampling zoonotic cutaneous leishmaniasis (ZCL) cases from patients and rodents. Biopsy samples were collected from 51 patients complaining of skin lesions, most of which (40 or 78.4%) proved to be ZCL. Amastigotes were detected in 33 patients (64.7%), but only 30 (58.9%) gave successful growth of promastigotes in the culture media. The positive cases were Saudis 14(35%) and non-Saudis 26 (65%). Five species of rodents were caught, Meriories libycus, Psammomys obesus, Rattus rattus, jaculus and Hystrix indica. The first species was the most dominant (90%) in which Leishmania parasites were detected. The Leishmania isolates from man and rodents were identified by isoenzyme electrophoresis and proved to be Zymodeme LON-4.
在沙特阿拉伯北部和西部的四个地区（麦地那·阿尔莫纳瓦拉、塔布克地区、焦夫地区和北部边境地区）开展了几次考察，以便从患者和啮齿动物中采集人畜共患皮肤利什曼病（ZCL）病例样本。从51名主诉有皮肤病变的患者身上采集了活检样本，其中大多数（40例，占78.4%）被证实为ZCL。在33名患者（64.7%）中检测到无鞭毛体，但只有30名（58.9%）在培养基中成功培养出前鞭毛体。阳性病例中沙特人有14例（35%），非沙特人有26例（65%）。捕获了五种啮齿动物，即利比亚沙鼠、肥沙鼠、黑家鼠、跳鼠和印度豪猪。第一种是最主要的（占90%），在其中检测到了利什曼原虫寄生虫。通过同工酶电泳鉴定了从人和啮齿动物分离出的利什曼原虫，结果证明是酶谱LON - 4。
Zotero 插件