酶联免疫吸附测定（ELISA）和间接血凝试验（IHAT）在确诊人类包虫病病例血清学诊断中的评估
Evaluation of ELISA and IHAT in serological diagnosis of proven cases of human hydatidosis.
作者信息
El-Shazly A M, Saad Ranya M, Belal Usama S, Sakr Tarek, Zakae Hytham A
机构信息
Department of Medical Parasitology, Faculty of Medicine, University of Mansoura, Saudi Arabia.
出版信息
J Egypt Soc Parasitol. 2010 Aug;40(2):531-8.
Cystic hydatid disease (Hydatidosis) is the most serious tape-worm infection prevalent in the cattle and sheep raising area of the world. Hydatidosis in man (as an accidental host) is caused by infection with the ova containing larval stage of Echinococcus granulosus. In the last decade, different techniques have been employed for sero-diagnosis of hydatidosis; as IHA, IFA, ELISA, CCLE (Counter Current Immuno-electrophoresis). This paper evaluated the validity of ELISA and IHA. Since whole hydatid cyst fluid was used as a source of antigen for serodiagnosis. Thirty surgical and pathological hydatidosis proven patients were examined. The sensitivity and specificity of ELISA were 96.7% & 97.5% respectively, and that of IHA were 86.7%, &95% respectively.
囊状棘球蚴病（包虫病）是世界上牛羊养殖地区流行的最严重的绦虫感染。人类（作为偶然宿主）的包虫病是由感染含有细粒棘球绦虫幼虫阶段的虫卵引起的。在过去十年中，已采用不同技术进行包虫病的血清学诊断；如间接血凝试验（IHA）、免疫荧光试验（IFA）、酶联免疫吸附测定（ELISA）、对流免疫电泳（CCLE）。本文评估了ELISA和IHA的有效性。由于整个棘球蚴囊液被用作血清学诊断的抗原来源。对30例经手术和病理证实为包虫病的患者进行了检查。ELISA的敏感性和特异性分别为96.7%和97.5%，IHA的敏感性和特异性分别为86.7%和95%。
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