Department of Parasitology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
Toxoplasmosis Research Center, Mazandaran University of Medical Sciences, Sari, Iran.
Infect Disord Drug Targets. 2024;24(7):e200224227165. doi: 10.2174/0118715265281114240131045945.
Cystic echinococcosis (CE) is a zoonotic parasitic disease caused by the metacestode of Echinococcus granulosus. CE is a health problem in Middle Eastern countries, such as Iran. The purpose of this study was to purify subunit 8 KDa antigen B from crude sheep hydatid cyst fluid (HCF) and compare its sensitivity and specificity with a commercial human ELISA kit (PT-Hydatid-96).
28 sera samples were collected from hydatid cyst patients who had surgery for a hydatid cyst and had their disease confirmed by pathology after the surgery. Furthermore, 35 samples of healthy individuals with no history of hydatid cysts were collected, as were nine serum samples from parasite-infected non-CE patients. HCF was obtained from sheep fertile cysts at a Sari slaughterhouse and used as an antigen. In an indirect ELISA test, the B antigen was employed, and the results were compared to those from a commercial ELISA kit.
The results of this study were analyzed using the Kappa test. The commercial ELISA kit showed 17 cases (23.6%) positive, 44 cases (61.1%) negative, and 11 cases (15.3%) borderline. B antigen showed that 18 (25%), 43 (59.7 %), and 11 (15.3%) were positive, negative, and borderline, respectively. One sample (1.4% of 72 total samples) of 35 serum samples from healthy individuals was positive using B antigen-based ELISA. In addition, all nine serum samples from parasite-infected non-CE patients were negative for both tests. The sensitivity and specificity of the commercial ELISA kit have been evaluated at 60.7% and 100%, respectively. For B antigenbased ELISA, these values are 64.3 and 97.7%, respectively.
Antigen B produced from hydatid cyst fluid is a promising option for serological identification of hydatid cysts in both infected and healthy individuals. In an indirect ELISA test, hydatid fluid antigen could be used as a precise source of detection.
包虫病（CE）是一种由细粒棘球绦虫的中绦期引起的人畜共患寄生虫病。CE 是中东国家（如伊朗）的一个健康问题。本研究的目的是从粗绵羊包虫囊液（HCF）中纯化亚单位 8 kDa 抗原 B，并将其与商业人类 ELISA 试剂盒（PT-Hydatid-96）进行比较，以评估其敏感性和特异性。
从接受包虫囊肿手术且术后病理证实患有包虫病的患者中采集 28 份血清样本。此外，采集了 35 份无包虫病史的健康个体样本和 9 份寄生虫感染非 CE 患者的血清样本。HCF 从 Sari 屠宰场的绵羊可育囊肿中获得，用作抗原。在间接 ELISA 试验中，使用 B 抗原，将结果与商业 ELISA 试剂盒进行比较。
本研究结果采用 Kappa 检验进行分析。商业 ELISA 试剂盒显示 17 例（23.6%）阳性、44 例（61.1%）阴性和 11 例（15.3%）边界。B 抗原显示分别有 18 例（25%）、43 例（59.7%）和 11 例（15.3%）阳性、阴性和边界。35 份健康个体血清样本中，有 1 份（72 份总样本的 1.4%）B 抗原 ELISA 阳性。此外，两种检测方法均对 9 份寄生虫感染非 CE 患者的血清样本均为阴性。商业 ELISA 试剂盒的敏感性和特异性分别为 60.7%和 100%。对于 B 抗原 ELISA，这些值分别为 64.3%和 97.7%。
从包虫囊液中提取的抗原 B 是感染和健康个体包虫病血清学鉴定的有前途的选择。在间接 ELISA 试验中，包虫液抗原可作为一种精确的检测源。
