Bremer C W
Biotechnology Division, ARC-Onderstepoort Veterinary Institute, Private Bag X05, Onderstepoort, 0110 South Africa.
J S Afr Vet Assoc. 2010 Jun;81(2):93-6. doi: 10.4102/jsava.v81i2.112.
About 90% of bovine malignant catarrhal fever (BMCF) PCR-positive cases in South Africa are caused by alcelaphine herpesvirus-1 (AlHV-1) and the other 10% by ovine herpesvirus-2 (OvHV-2). The prevalence of OvHV-2 in different sheep breeds in South Africa was determined in order to investigate whether the lower incidence of BMCF caused by OvHV-2 in comparison with AlHV-1 can be ascribed to a low incidence of the virus in sheep. A single-tube hemi-nested PCR was developed, evaluated and applied to detect OvHV-2 DNA. The prevalence of the virus in 4 sheep breeds from various regions in South Africa was shown to be 77%. No statistically significant difference was found amongst the sheep breeds tested.
在南非,约90%的牛恶性卡他热(BMCF)PCR阳性病例由非洲马瘟病毒1型(AlHV-1)引起,另外10%由绵羊疱疹病毒2型(OvHV-2)引起。为了调查与AlHV-1相比,OvHV-2引起的BMCF发病率较低是否可归因于该病毒在绵羊中的低发病率,对南非不同绵羊品种中OvHV-2的流行情况进行了测定。开发、评估并应用了一种单管半巢式PCR来检测OvHV-2 DNA。结果显示,南非不同地区4个绵羊品种中该病毒的流行率为77%。在所检测的绵羊品种之间未发现统计学上的显著差异。