Mataveia G A, Terblanche S J, Nöthling J O, Gerber D
Section of Reproduction, Department of Clinic, Veterinary Faculty, Eduardo Mondlane University, P O Box 257, Maputo, Mozambique.
J S Afr Vet Assoc. 2010 Sep;81(3):139-42. doi: 10.4102/jsava.v81i3.135.
Ram seminal plasma increases the fertility of frozen-thawed ram spermatozoa deposited into the cervix. The aim of the current study was to compare the effect of ram seminal plasma to that of bull seminal plasma, dog prostatic fluid, protein-free TALP TrilEq (Triladyl with 0.5 mt of Equex STM paste added to each 100 mt) and heat-treated skim milk on longevity and percentages of progressively motile and aberrantly motile frozen-thawed ram spermatozoa. Three ejaculates from each of 6 rams were extended in TrilEq, pooled and frozen in straws as a single batch per ram. One hundred and eight straws (3 straws from each ram for each fluid) were thawed in random order. Once thawed, a straw was emptied into a tube with 0.85 ml of the appropriate fluid at 37 degrees C and kept at that temperature for 6 h. Motility was assessed at x200 magnification immediately (time zero) and 2, 4 and 6 h after thawing. Progressive motility decreased from each time to the next (P < 0.05) and was 39.0 % (0 h), 26.0 % (2 h), 19.6 % (4 h) and 12.6 % (6 h); SEM 1.24, n = 108 for each group. Ram seminal plasma resulted in higher progressive motility than bull seminal plasma, lower than milk, and similar to the other fluids. Ram seminal plasma resulted in lower aberrant motility than protein-free TALP and similar aberrant motility to other fluids. The effect of ram seminal plasma and dog prostatic fluid was very similar. The effect of ram seminal plasma on the fertility of frozen-thawed ram spermatozoa deposited into the cervix is not due an exceptionally beneficial effect on the motility of spermatozoa.
公羊精浆可提高冻融后存入子宫颈的公羊精子的受精能力。本研究的目的是比较公羊精浆与公牛精浆、犬前列腺液、无蛋白TALP TrilEq（每100毫升Triladyl中添加0.5毫升Equex STM糊剂）以及热处理脱脂奶对冻融后公羊精子的存活时间、进行性运动精子百分比和异常运动精子百分比的影响。从6只公羊中每只采集3次射精，用TrilEq进行稀释，合并后按每只公羊作为一批次冷冻于细管中。108根细管（每种液体从每只公羊取3根细管）按随机顺序解冻。解冻后，将一根细管中的精液倒入含有0.85毫升相应液体的试管中，于37℃保存6小时。在x200放大倍数下立即（时间0）以及解冻后2、4和6小时评估精子活力。进行性运动精子百分比随时间逐渐下降（P<0.05），分别为39.0%（0小时）、26.0%（2小时）、19.6%（4小时）和12.6%（6小时）；每组标准误为1.24，n = 108。公羊精浆组的进行性运动精子百分比高于公牛精浆组，低于牛奶组，与其他液体组相似。公羊精浆组的异常运动精子百分比低于无蛋白TALP组，与其他液体组相似。公羊精浆和犬前列腺液的作用非常相似。公羊精浆对冻融后存入子宫颈的公羊精子受精能力的影响并非源于对精子活力的特别有益作用。
