Benmoula Anass, Badi Abdelmoughit, El Fadili Moussa, El Khalil Kaoutar, Allai Larbi, El Hilali Abderaouf, El Amiri Bouchra
INRA-Centre Régional de la Recherche Agronomique de Settat, BP589, Settat, Morocco; Laboratoire d'agroalimentaire et santé, Faculté des Sciences et Techniques, Université Hassan1, BP 577, 26000 Settat, Morocco.
INRA-Centre Régional de la Recherche Agronomique de Settat, BP589, Settat, Morocco.
Anim Reprod Sci. 2017 May;180:17-22. doi: 10.1016/j.anireprosci.2017.02.008. Epub 2017 Feb 22.
The present study was undertaken to assess the effect of seasons on scrotal circumference, semen characteristics, seminal plasma composition, and sperm motility during liquid storage of INRA180 rams. The semen was collected from five mature INRA180 rams (2-3 years of age) during one year (from April 2014 to March 2015). Scrotal circumferences, semen characteristics, some biochemical parameters of seminal plasma were evaluated. Immediately after collection and evaluation, the semen was pooled and extended in skim milk (SM) at 15°C to reach 0.8×109 spermatozoa/ml. Thereafter, samples were evaluated at different storage times (0, 8, and 24h). The results showed that scrotal circumference, semen quality and the concentration of total protein in seminal plasma were relatively constant during the year (P>0.05). However, total lipid and cholesterol concentrations increased significantly (P<0.001) in winter and summer. The result showed also that progressive motility was higher in winter and summer after 24h of storage (P<0.01). In contrast, no difference was recorded regarding total motility (P>0.05). To conclude, the INRA180 rams have the ability to produce semen with high quality all over the year. The only parameters showing seasonal variations are cholesterol, total lipid, and progressive motility.
本研究旨在评估季节对INRA180公羊精液液态保存期间阴囊周长、精液特征、精浆成分及精子活力的影响。在一年时间内（2014年4月至2015年3月）从5只成熟的INRA180公羊（2 - 3岁）采集精液。评估阴囊周长、精液特征以及精浆的一些生化参数。采集并评估后，立即将精液混合，在15°C条件下用脱脂乳（SM）稀释至0.8×10⁹精子/ml。此后，在不同储存时间（0、8和24小时）对样本进行评估。结果表明，一年中阴囊周长、精液质量和精浆中总蛋白浓度相对稳定（P>0.05）。然而，冬季和夏季总脂质和胆固醇浓度显著增加（P<0.001）。结果还表明，储存24小时后，冬季和夏季的前向运动率较高（P<0.01）。相比之下，总活力方面无差异（P>0.05）。总之，INRA180公羊全年都有能力产生高质量的精液。仅显示出季节性变化的参数是胆固醇、总脂质和前向运动率。
