National Institute of Advanced Industrial Science and Technology, Takamatsu 761-0395, Japan.
J Pharm Biomed Anal. 2011 Apr 28;55(1):202-5. doi: 10.1016/j.jpba.2010.12.014. Epub 2010 Dec 21.
A highly sensitive DNA detection method using a combination of ethidium bromide (EtBr) and SYBR Green II (SG II) for microchip electrophoresis was developed. By use of the combination of these intercalating DNA-staining dyes for microchip electrophoresis with Hitachi SV1100 system, the fluorescence intensities corresponding to DNA fragments were obviously increased over those obtained with EtBr only, with accuracy of DNA sizing and quantification. The detection limit with EtBr and the combination of EtBr and SG II were 0.048 and 0.007ng/μl, respectively. This highly sensitive DNA detection just using the combination of these dyes offering high resolution in a short time will be useful for various biological analyses.
采用溴化乙锭（EtBr）和 SYBR Green II（SG II）联合的方法，开发了一种用于微芯片电泳的高灵敏度 DNA 检测方法。通过在日立 SV1100 系统上使用这些嵌入性 DNA 染色染料的组合进行微芯片电泳，与仅使用 EtBr 相比，相应的 DNA 片段的荧光强度明显增加，从而实现了 DNA 大小和定量的准确性。使用 EtBr 和 EtBr 和 SG II 的组合的检测限分别为 0.048ng/μl 和 0.007ng/μl。这种仅使用这些染料联合的高灵敏度 DNA 检测方法在短时间内提供高分辨率，将有助于各种生物分析。
