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噬菌体phi 29转录激活蛋白的N端短缺失会损害其DNA结合能力。

Short N-terminal deletions in the phage phi 29 transcriptional activator protein impair its DNA-binding ability.

作者信息

Rojo F, Salas M

机构信息

Centro de Biología Molecular (CSIC-UAM), Universidad Autónoma de Madrid, Spain.

出版信息

Gene. 1990 Nov 30;96(1):75-81. doi: 10.1016/0378-1119(90)90343-p.

DOI:10.1016/0378-1119(90)90343-p
PMID:2125015
Abstract

The expression of Bacillus subtilis phage phi 29 late genes from the A3 promoter requires the viral protein p4. This protein is a transcriptional activator which binds to a region of the A3 promoter located between nucleotides -56 to -102, relative to the transcription start point. Mutants at the N terminus of protein p4 have been constructed and their function investigated. The binding of these deletion mutants to the late A3 promoter has been analyzed by gel retardation and DNase I footprinting assays. The results indicate that the N terminus of protein p4 could be involved in its binding to the A3 promoter, suggesting that it may not be a typical Cro-like helix-turn-helix DNA-binding protein.

摘要

来自枯草芽孢杆菌噬菌体φ29的晚期基因在A3启动子处的表达需要病毒蛋白p4。该蛋白是一种转录激活因子，它结合到相对于转录起始点核苷酸-56至-102之间的A3启动子区域。已构建了蛋白p4 N端的突变体并对其功能进行了研究。通过凝胶阻滞和DNase I足迹分析来分析这些缺失突变体与晚期A3启动子的结合。结果表明，蛋白p4的N端可能参与其与A3启动子的结合，这表明它可能不是典型的Cro样螺旋-转角-螺旋DNA结合蛋白。

相似文献

1
Short N-terminal deletions in the phage phi 29 transcriptional activator protein impair its DNA-binding ability.噬菌体phi 29转录激活蛋白的N端短缺失会损害其DNA结合能力。
Gene. 1990 Nov 30;96(1):75-81. doi: 10.1016/0378-1119(90)90343-p.
2
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