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Effects of internal deletions on the priming activity of the phage phi 29 terminal protein.

作者信息

Zaballos A, Lázaro J M, Méndez E, Mellado R P, Salas M

机构信息

Centro de Biología Molecular, (CSIC-UAM), Universidad Autónoma, Madrid, Spain.

出版信息

Gene. 1989 Nov 30;83(2):187-95. doi: 10.1016/0378-1119(89)90104-2.

DOI:10.1016/0378-1119(89)90104-2
PMID:2511080
Abstract

A series of internal deletions of gene 3, coding for the phage phi 29 DNA terminal protein, have been constructed and characterized. In addition, a substitution mutant in the sequence corresponding to amino acids (aa) 49-51 was obtained. The priming activity of the substitution mutant protein, in the formation of the protein p3-dAMP initiation complex, was drastically reduced suggesting that some of the aa present at position 49-51 are essential for p3 function. Deletions of 8 to 33 aa, from aa residue 48 towards the N terminus of the substitution mutant, further decreased the priming activity of the protein. The activity of deletion mutants lacking 15 or 21 aa from residue 57 towards the C terminus, and also containing a point mutation at position 56, was greatly reduced, and no activity was seen when 24 aa were lacking.

摘要

相似文献

1
Effects of internal deletions on the priming activity of the phage phi 29 terminal protein.
Gene. 1989 Nov 30;83(2):187-95. doi: 10.1016/0378-1119(89)90104-2.
2
Characterization, overproduction and purification of the product of gene 1 of Bacillus subtilis phage phi 29.枯草芽孢杆菌噬菌体φ29基因1产物的特性鉴定、过量表达及纯化
Gene. 1989 Apr 30;77(2):195-204. doi: 10.1016/0378-1119(89)90067-x.
3
Initiation of phage phi 29 DNA replication by mutants with deletions at the carboxyl end of the terminal protein.末端蛋白羧基端缺失突变体引发噬菌体φ29 DNA复制的研究
Gene. 1986;43(1-2):103-10. doi: 10.1016/0378-1119(86)90013-2.
4
Initiation of phage phi 29 DNA replication by mutants with deletions at the amino end of the terminal protein.通过在末端蛋白氨基端有缺失的突变体启动噬菌体φ29 DNA复制。
Gene. 1988;63(1):113-21. doi: 10.1016/0378-1119(88)90550-1.
5
Cloning and expression in Escherichia coli of the gene coding for the protein linked to the ends of Bacillus subtilis phage phi 29 DNA.枯草芽孢杆菌噬菌体 phi 29 DNA 末端连接蛋白编码基因在大肠杆菌中的克隆与表达。
Gene. 1983 Jan-Feb;21(1-2):65-76. doi: 10.1016/0378-1119(83)90148-8.
6
Short N-terminal deletions in the phage phi 29 transcriptional activator protein impair its DNA-binding ability.噬菌体phi 29转录激活蛋白的N端短缺失会损害其DNA结合能力。
Gene. 1990 Nov 30;96(1):75-81. doi: 10.1016/0378-1119(90)90343-p.
7
High level synthesis in Escherichia coli of the Bacillus subtilis phage phi 29 proteins p3 and p4 under the control of phage lambda PL promoter.在噬菌体λ PL 启动子的控制下，枯草芽孢杆菌噬菌体 phi 29 蛋白 p3 和 p4 在大肠杆菌中的高水平合成。
Nucleic Acids Res. 1982 Oct 11;10(19):5773-84. doi: 10.1093/nar/10.19.5773.
8
Cloning and expression of gene 2, required for the protein-primed initiation of the Bacillus subtilis phage phi 29 DNA replication.枯草芽孢杆菌噬菌体phi 29 DNA复制蛋白引发起始所必需的基因2的克隆与表达。
Gene. 1984 Jul-Aug;29(1-2):33-40. doi: 10.1016/0378-1119(84)90163-x.
9
Characterization of the phage phi 29 protein p5 as a single-stranded DNA binding protein. Function in phi 29 DNA-protein p3 replication.噬菌体φ29蛋白p5作为单链DNA结合蛋白的特性。在φ29 DNA-蛋白p3复制中的功能。
Nucleic Acids Res. 1989 May 25;17(10):3663-72. doi: 10.1093/nar/17.10.3663.
10
Initiation of phage phi 29 DNA replication by the terminal protein modified at the carboxyl end.由羧基末端修饰的末端蛋白启动噬菌体φ29 DNA复制。
Nucleic Acids Res. 1983 Nov 11;11(21):7397-407. doi: 10.1093/nar/11.21.7397.

引用本文的文献

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Engineering Permissive Insertion Sites in the Bacteriophage Phi29 DNA-Linked Terminal Protein.在噬菌体Phi29 DNA连接末端蛋白中构建允许插入位点
PLoS One. 2016 Oct 25;11(10):e0164901. doi: 10.1371/journal.pone.0164901. eCollection 2016.
2
Involvement of the TPR2 subdomain movement in the activities of phi29 DNA polymerase.TPR2亚结构域运动在phi29 DNA聚合酶活性中的作用。
Nucleic Acids Res. 2009 Jan;37(1):193-203. doi: 10.1093/nar/gkn928. Epub 2008 Nov 25.
3
Involvement of phage phi29 DNA polymerase and terminal protein subdomains in conferring specificity during initiation of protein-primed DNA replication.
噬菌体phi29 DNA聚合酶和末端蛋白亚结构域在蛋白质引发的DNA复制起始过程中赋予特异性的作用。
Nucleic Acids Res. 2007;35(21):7061-73. doi: 10.1093/nar/gkm749. Epub 2007 Oct 2.
4
Involvement of phi29 DNA polymerase thumb subdomain in the proper coordination of synthesis and degradation during DNA replication.phi29 DNA聚合酶拇指亚结构域在DNA复制过程中合成与降解的适当协调中的作用。
Nucleic Acids Res. 2006 Jun 6;34(10):3107-15. doi: 10.1093/nar/gkl402. Print 2006.
5
The phi29 DNA polymerase:protein-primer structure suggests a model for the initiation to elongation transition.phi29 DNA聚合酶：蛋白质引物结构为起始到延伸转变提出了一个模型。
EMBO J. 2006 Mar 22;25(6):1335-43. doi: 10.1038/sj.emboj.7601027. Epub 2006 Mar 2.
6
Phi29 DNA polymerase residues Tyr59, His61 and Phe69 of the highly conserved ExoII motif are essential for interaction with the terminal protein.高度保守的ExoII基序的Phi29 DNA聚合酶残基Tyr59、His61和Phe69对于与末端蛋白的相互作用至关重要。
Nucleic Acids Res. 2002 Mar 15;30(6):1379-86. doi: 10.1093/nar/30.6.1379.
7
Compartmentalization of phage phi29 DNA replication: interaction between the primer terminal protein and the membrane-associated protein p1.噬菌体phi29 DNA复制的区室化：引物末端蛋白与膜相关蛋白p1之间的相互作用。
EMBO J. 2000 Oct 16;19(20):5575-84. doi: 10.1093/emboj/19.20.5575.
8
Bacteriophage phi29 DNA replication arrest caused by codirectional collisions with the transcription machinery.由与转录机制的同向碰撞导致的噬菌体phi29 DNA复制停滞。
EMBO J. 1997 Sep 15;16(18):5775-83. doi: 10.1093/emboj/16.18.5775.
9
Primer-terminus stabilization at the 3'-5' exonuclease active site of phi29 DNA polymerase. Involvement of two amino acid residues highly conserved in proofreading DNA polymerases.引物末端在phi29 DNA聚合酶3'-5'核酸外切酶活性位点的稳定作用。两个在具有校对功能的DNA聚合酶中高度保守的氨基酸残基的作用。
EMBO J. 1996 Mar 1;15(5):1182-92.
10
Functional domains in the bacteriophage phi 29 terminal protein for interaction with the phi 29 DNA polymerase and with DNA.噬菌体φ29末端蛋白中与φ29 DNA聚合酶及DNA相互作用的功能结构域。
Nucleic Acids Res. 1989 Dec 25;17(24):10353-66. doi: 10.1093/nar/17.24.10353.