细胞松弛素D对小鼠脾细胞中干扰素产生的抑制作用。

Suppression of interferon production in mouse spleen cells by cytochalasin D.

作者信息

Ito Y, Nishiyama Y, Shimokata K, Takeyama H, Kunii A

出版信息

J Gen Virol. 1978 Oct;41(1):129-34. doi: 10.1099/0022-1317-41-1-129.

Abstract

Cytochalasin D is thought to impair microfilament function. The present study has investigated its effects on four different systems in which interferon is formed, namely (1) mouse fibroblasts induced with virus (2) mouse spleen cells induced with virus, or (3) with endotoxin or (4) by allogeneic stimulation. Cytochalasin D did not suppress formation of interferon by fibroblasts (L cells) or spleen cells stimulated with either HVJ or NDV. However it did suppress production of interferon by spleen cells in response to endotoxin or an allogeneic stimulation; here its action was apparently not on the secretion of interferon, but on some earlier event. It also suppressed the production of interferon by mouse spleen cells induced with HVJ if this had been u.v. irradiated for more than 15 min: this suggests that cytochalasin D sensitive structures do play some role in interferon production by mouse spleen cells when stimulated with HVJ, as well as when they are stimulated with endotoxin or an allogeneic stimulus.

摘要

细胞松弛素D被认为会损害微丝功能。本研究调查了其对产生干扰素的四种不同系统的影响，即：（1）用病毒诱导的小鼠成纤维细胞；（2）用病毒诱导的小鼠脾细胞；（3）用内毒素诱导的小鼠脾细胞；或（4）通过同种异体刺激诱导的小鼠脾细胞。细胞松弛素D不会抑制由HVJ或NDV刺激的成纤维细胞（L细胞）或脾细胞产生干扰素。然而，它确实会抑制脾细胞对内毒素或同种异体刺激产生干扰素；在这里，其作用显然不是针对干扰素的分泌，而是针对一些更早的事件。如果用HVJ诱导的小鼠脾细胞经紫外线照射超过15分钟，细胞松弛素D也会抑制其产生干扰素：这表明，细胞松弛素D敏感结构在小鼠脾细胞被HVJ刺激时以及被内毒素或同种异体刺激时产生干扰素的过程中确实发挥了一定作用。