Xiao Zheng-Xiang, Yin Xiao-Cheng, Tan Yan-Fang, Peng Yan-Hui
First Affiliated Hospital of Nanhua University, Hengyang, Hunan 421001, China.
Zhongguo Dang Dai Er Ke Za Zhi. 2011 Jan;13(1):53-6.
To study the effects of diallyl disulfide (DADS) on apoptosis of human leukemia K562 cells and possible mechanisms.
The morphologic changes of leukemia K562 cells after DADS treatment were observed by Hoechst 33258 staining. Cell apoptosis rates after different concentrations and different durations of DADS treatment were determined by flow cytometry. Fas, FasL and caspase-8 mRNA expression was estimated by reverse transcription-polymerase chain reaction (RT-PCR) 48 hrs after DADS treatment.
The characteristics of apoptosis in K562 cells induced by DADS were observed. After 24 hrs of DADS treatment, the apoptosis rate of K562 cells increased from (11.60 ± 0.83)% at the concentration of 10 mg/L to (37.94 ± 0.87)% at the concentration of 40 mg/L. The apoptosis rate of K562 cells increased after 40 mg/L DADS with the increasing time from (37.94 ± 0.87)% (24 hrs) to (47.02 ± 0.66)% (72 hrs). Expression of Fas and caspase-8 mRNA increased, while FasL mRNA expression decreased significantly 48 hrs after DADS treatment compared with the control group (P<0.05).
DADS can induce apoptosis of human leukemia K562 cells in a time- and concentration-dependent manner, possibly through increasing Fas and caspase-8 expression and decreasing FasL expression.
研究二烯丙基二硫醚（DADS）对人白血病K562细胞凋亡的影响及其可能机制。
采用Hoechst 33258染色观察DADS处理后白血病K562细胞的形态学变化。通过流式细胞术检测不同浓度和不同作用时间的DADS处理后细胞凋亡率。在DADS处理48小时后，采用逆转录-聚合酶链反应（RT-PCR）检测Fas、FasL和caspase-8 mRNA表达。
观察到DADS诱导K562细胞凋亡的特征。DADS处理24小时后，K562细胞凋亡率从10mg/L浓度时的（11.60±0.83）%增加到40mg/L浓度时的（37.94±0.87）%。40mg/L DADS处理后，随着时间延长，K562细胞凋亡率从（37.94±0.87）%（24小时）增加到（47.02±0.66）%（72小时）。与对照组相比，DADS处理48小时后Fas和caspase-8 mRNA表达增加，而FasL mRNA表达显著降低（P<0.05）。
DADS可呈时间和浓度依赖性诱导人白血病K562细胞凋亡，其机制可能是通过增加Fas和caspase-8表达以及降低FasL表达来实现。
