Darai G, Flugel R M
J Gen Virol. 1978 Oct;41(1):179-81. doi: 10.1099/0022-1317-41-1-179.
Mink lung cells (MvILu) are highly susceptible to varicella-zoster virus (VZV). The titres of cell-free VZV suspensions reached 1.0 x 10(7) p.f.u./ml at 3 days post-infection, with subsequent cell degeneration, if MvILu cells were infected with a multiplicity of infectious virus of 0.01 p.f.u./cell. In contrast, during the same period and under the same conditions the titres of cell-free VZV were 10(2) to 10(3) times lower when grown on human foreskin fibroblasts. A fast and reliable plaque assay and a neutralization test for VZV on MvILU cells, were developed.
水貂肺细胞(MvILu)对水痘-带状疱疹病毒(VZV)高度敏感。如果以0.01个蚀斑形成单位/细胞的感染复数感染MvILu细胞,在感染后3天,无细胞VZV悬液的滴度可达1.0×10⁷蚀斑形成单位/毫升,随后细胞发生变性。相比之下,在相同时间和相同条件下,在人包皮成纤维细胞上生长时,无细胞VZV的滴度要低10²至10³倍。开发了一种用于VZV在MvILU细胞上的快速可靠蚀斑测定法和中和试验。