恒河猴水痘病毒产量的提高及检测，以及恒河猴和人水痘病毒某些生物学特性的比较。

J Virol Methods. 1982 Nov;5(3-4):229-41. doi: 10.1016/0166-0934(82)90013-1.

Studies were performed to define conditions under which propagation, assay and stabilization of the Delta herpesvirus (DHV) strain of simian varicella virus might be improved, and to compare biological properties of DHV with those of human varicella zoster virus (VZV). A mycoplasma contaminant was successfully eliminated from the DHV seed virus by treatment with a specific anti-serum. DHV was found to replicate more efficiently in the BS-C-1 line of African green monkey kidney cells than in Vero cells, and seed virus preparations in the form of virus-infected cells were produced which had infectivity titers greater than or equal to 1 X 10(6) p.f.u./ml. Greater yields of virus were produced in cultures infected as dispersed cells than as preformed monolayers. Infectious DHV could be released from host cells by sonic treatment of heavily infected cultures at 48 h post infection. Certain agents reported to enhance replication of herpes viruses (caffeine, carbaryl, the tumor promoter 12-0-tetra-decanoyl-phorbol-13-acetate, and DEAE-dextran) had no enhancing effect on replication of DHV. However, DEAE-dextran in the maintenance medium enhanced spontaneous release of DHV into culture fluids. Plaquing efficiency and plaque size of DHV were greater in BS-C-1 than in Vero cells, and plaque assays and plaque reduction neutralization tests were developed in this cell system using a solid overlay medium with neutral red vital stain. Neutralization of DHV was markedly enhanced by fresh guinea pig complement. The newly developed neutralization test demonstrated more vigorous antibody responses to DHV in active and latent VZV infections than were demonstrated with previous procedures. In addition to their preferential growth in monkey and human cells respectively, DHV and VZV were found to differ markedly in their rates of attachment to host cells, with DHV requiring over 6 h of adsorption, while VZV adsorption was essentially complete at 1 h. Also, cell-free DHV was much more resistant than cell-free VZV to repeated cycles of freezing and thawing.

开展了多项研究，以确定可改善猴水痘病毒的δ疱疹病毒（DHV）毒株的增殖、检测及稳定性的条件，并比较DHV与人水痘带状疱疹病毒（VZV）的生物学特性。通过用特异性抗血清处理，成功地从DHV种子病毒中清除了支原体污染物。发现DHV在非洲绿猴肾细胞的BS-C-1系中比在Vero细胞中复制效率更高，并制备了病毒感染细胞形式的种子病毒制剂，其感染性滴度大于或等于1×10(6) p.f.u./ml。以分散细胞形式感染的培养物比预先形成的单层培养物产生的病毒产量更高。感染48小时后，通过对感染严重的培养物进行超声处理，可从宿主细胞中释放出有感染性的DHV。据报道，某些可增强疱疹病毒复制的试剂（咖啡因、西维因、肿瘤促进剂12-0-十四烷酰佛波醇-13-乙酸酯和DEAE-葡聚糖）对DHV的复制没有增强作用。然而，维持培养基中的DEAE-葡聚糖可增强DHV向培养液中的自发释放。DHV在BS-C-1细胞中的空斑形成效率和空斑大小均高于Vero细胞，并在该细胞系统中使用含有中性红活体染料的固体覆盖培养基开展了空斑试验和空斑减少中和试验。新鲜豚鼠补体可显著增强对DHV的中和作用。新开发的中和试验显示，与先前的方法相比，在活动性和潜伏性VZV感染中，对DHV的抗体反应更为强烈。除了分别在猴细胞和人细胞中优先生长外，还发现DHV和VZV在与宿主细胞的附着速率上有显著差异，DHV需要超过6小时的吸附时间，而VZV在1小时时吸附基本完成。此外，无细胞的DHV比无细胞的VZV对反复冻融循环的耐受性要强得多。