Department of Immunology and Physiology, College of Medicine, Konkuk University, Chungju, Republic of Korea.
J Ethnopharmacol. 2011 Jul 14;136(3):392-8. doi: 10.1016/j.jep.2011.01.016. Epub 2011 Jan 18.
Morus bombycis Koidzumi is widely distributed in Asia. In Korea, it has been used in traditional medicine because of its apparent anti-inflammatory, antioxidant, and hepatoprotective properties.
Although the extract of Morus bombycis Koidzumi (MB) has long since been used as a traditional anti-inflammatory medicine in Korea, its effect on arthritis remains unknown. We aimed to investigate the anti-arthritis activity of MB and the mechanism underlying it.
The anti-arthritis activity of MB was assessed by using mouse models of type II collagen-induced arthritis (CIA). The clinical arthritis index and histopathological changes were evaluated in mice. Reverse transcriptase-polymerase chain reaction (RT-PCR), electrophoretic mobility shift assay (EMSA), and other biologic approaches were used for measuring the effect of MB on arthritis and understanding the underlying mechanism.
MB significantly decreased the clinical arthritis index in CIA mice; this was confirmed by examining histological changes in joints. Infiltration of immune cells, synovial hyperplasia, cartilage destruction, and bone erosion in the hind paw were largely suppressed by MB. The mRNA levels of matrix metalloproteinase (MMP)-1/MMP-3, inflammatory cytokines (tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-6), and chemokines (macrophage inflammatory protein (MIP)-1, monocyte chemoattractant protein (MCP)-1, RANTES) were significantly suppressed by MB in a dose-dependent manner. The number of osteoclasts in the hind tibia was also significantly decreased. With regard to the mechanism, MB suppressed the activation of nuclear factor (NF)-κB and activator protein (AP)-1 in CIA mice.
MB produced an anti-arthritis effect in CIA mice by inhibiting the production of critical inflammatory mediators and osteoclasts through the downregulation of NF-κB and AP-1.
桑黄在亚洲广泛分布。在韩国，由于其明显的抗炎、抗氧化和保肝作用，它被用于传统医学。
虽然桑黄提取物（MB）早已在韩国被用作传统抗炎药物，但它对关节炎的作用仍不清楚。我们旨在研究 MB 的抗关节炎活性及其作用机制。
采用 II 型胶原诱导关节炎（CIA）小鼠模型评估 MB 的抗关节炎活性。在小鼠中评估临床关节炎指数和组织病理学变化。采用逆转录-聚合酶链反应（RT-PCR）、电泳迁移率变动分析（EMSA）和其他生物学方法来测量 MB 对关节炎的影响并了解其潜在机制。
MB 显著降低 CIA 小鼠的临床关节炎指数；通过检查关节的组织学变化得到证实。MB 大量抑制免疫细胞浸润、滑膜增生、软骨破坏和后爪骨侵蚀。MB 还以剂量依赖性方式显著抑制基质金属蛋白酶（MMP）-1/MMP-3、炎症细胞因子（肿瘤坏死因子（TNF）-α、白细胞介素（IL）-1β、IL-6）和趋化因子（巨噬细胞炎症蛋白（MIP）-1、单核细胞趋化蛋白（MCP）-1、调节激活正常 T 细胞表达和分泌因子（RANTES））的 mRNA 水平。后胫骨中的破骨细胞数量也显著减少。关于机制，MB 通过抑制核因子（NF）-κB 和激活蛋白（AP）-1 的激活，抑制关键炎症介质和破骨细胞的产生，从而在 CIA 小鼠中产生抗关节炎作用。
MB 通过下调 NF-κB 和 AP-1 抑制关键炎症介质和破骨细胞的产生，对 CIA 小鼠产生抗关节炎作用。
