Peterson D A, Wolfe L G, Larkin E P, Deinhardt F W
J Med Virol. 1978;2(3):201-6. doi: 10.1002/jmv.1890020303.
The susceptibility of white-lipped marmoset monkeys (Saguinus sp) to human hepatitis A virus (HAV) provides a system for evaluation of thermal inactivation of HAV in feces and contaminated shellfish. Intramuscular or oral administration of HAV derived from feces of four patients with acute hepatitis A induced hepatitis in 28--100% of the inoculated marmosets. A 10% (w/v) fecal pool (GBG-BM) prepared from two patients (GBG and GBM) induced hepatitis in marmosets (2/4 with 1 ml; 2/2 with 3 ml) when given orally as a 1 : 3 dilution. A HAV-baby food raw oyster mixture fed to fasted marmosets induced hepatitis in 1/4 and seroconversion in 2/4 animals. Two groups of oysters were injected with HAV (concentrated 3 : 1 by centrifugation of the GBG-BM pool); one group was treated at 140 degrees F for 19 minutes and the other served as an untreated control. In animals fed the untreated inoculum, 4/6 developed hepatitis and 6/6 seroconverted, whereas of those fed the heat-treated inoculum 1/7 developed hepatitis and 2/7 seroconverted. These data suggest that pasteurization methods could be developed that would eliminate shellfish-associated hepatitis A and retain the palatability of the shellfish.
白唇狨猴（Saguinus sp）对人甲型肝炎病毒（HAV）的易感性为评估粪便和受污染贝类中HAV的热灭活提供了一个系统。对28 - 100%接种的狨猴进行肌肉注射或口服来自4例急性甲型肝炎患者粪便的HAV可诱发肝炎。由两名患者（GBG和GBM）制备的10%（w/v）粪便混合液（GBG - BM）以1:3稀释口服给药时，可使狨猴发生肝炎（1毫升剂量组2/4发病；3毫升剂量组2/2发病）。给禁食的狨猴喂食含有HAV的婴儿食品生牡蛎混合物，1/4动物发生肝炎，2/4动物出现血清转化。两组牡蛎注射HAV（通过GBG - BM混合液离心浓缩3:1）；一组在140华氏度处理19分钟，另一组作为未处理对照。在喂食未处理接种物的动物中，4/6发生肝炎，6/6出现血清转化，而在喂食热处理接种物的动物中，1/7发生肝炎，2/7出现血清转化。这些数据表明，可以开发巴氏杀菌方法，既能消除与贝类相关的甲型肝炎，又能保持贝类的适口性。
