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Small interfering RNA (siRNA): a hope for the loss-of-function studies in anesthesiology?

作者信息

Kim Yun Hong

机构信息

Department of Anesthesiology and Pain Medicine, Kangbuk Samsung Hospital, Sungkyunkwan University School of Medicine, Seoul, Korea.

出版信息

Korean J Anesthesiol. 2010 Dec;59(6):369-70. doi: 10.4097/kjae.2010.59.6.369. Epub 2010 Dec 31.

Abstract
摘要

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The optimal concentration of siRNA for gene silencing in primary cultured astrocytes and microglial cells of rats.
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Suppression of heart NF-κB p65 expression by jugular vein injection of RNAi in mice.
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In vivo target validation using gene invalidation, RNA interference and protein functional knockout models: it is the time to combine.
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