Macedo Gilson C, Bozzi Adriana, Weinreich Helena Rachel, Bafica Andre, Teixeira Henrique C, Oliveira Sergio C
Laboratory of Immunology of Infectious Diseases, Department of Biochemistry and Immunology, Institute of Biological Sciences, Federal University of Minas Gerais, 31270-901 Belo Horizonte, MG, Brazil.
Clin Dev Immunol. 2011;2011:351573. doi: 10.1155/2011/351573. Epub 2010 Dec 29.
Tuberculosis remains a major health problem throughout the world causing large number of deaths. Effective disease control and eradication programs require the identification of major antigens recognized by the protective responses against M. tuberculosis. In this study, we have investigated humoral and cellular immune responses to M. tuberculosis-specific Ag85A, Ag85B, and ESAT-6 antigens in Brazilian patients with pulmonary (P, n = 13) or extrapulmonary (EP, n = 12) tuberculosis, patients undergoing chemotherapy (PT, n = 23), and noninfected healthy individuals (NI, n = 7). Compared to NI, we observed increased levels of IgG1 responses to Ag85B and ESAT-6 in P and PT groups. Regarding cellular immunity, Ag85A and ESAT-6 were able to discriminate P, PT, and EP patients from healthy individuals by IFN-γ production and P and PT groups from EP individuals by production of TNF-α. In summary, these findings demonstrate the ability of Ag85A, Ag85B, and ESAT-6 to differentiate TB patients from controls by IgG1, IFN-γ and TNF-α production.
结核病仍然是全球主要的健康问题，导致大量死亡。有效的疾病控制和根除计划需要识别出针对结核分枝杆菌的保护性反应所识别的主要抗原。在本研究中，我们调查了巴西肺结核（P组，n = 13）或肺外结核（EP组，n = 12）患者、接受化疗的患者（PT组，n = 23）以及未感染的健康个体（NI组，n = 7）对结核分枝杆菌特异性Ag85A、Ag85B和ESAT-6抗原的体液免疫和细胞免疫反应。与NI组相比，我们观察到P组和PT组中针对Ag85B和ESAT-6的IgG1反应水平升高。关于细胞免疫，Ag85A和ESAT-6能够通过干扰素-γ的产生将P组、PT组和EP组患者与健康个体区分开来，并通过肿瘤坏死因子-α的产生将P组和PT组与EP组个体区分开来。总之，这些发现表明Ag85A、Ag85B和ESAT-6能够通过IgG1、干扰素-γ和肿瘤坏死因子-α的产生将结核病患者与对照组区分开来。
