Institute of Microbiology of the ASCR, v.v.i., 14220 Praha, Czech Republic.
Folia Microbiol (Praha). 2010 Nov;55(6):662-5. doi: 10.1007/s12223-010-0107-5. Epub 2011 Jan 21.
A new method providing a relief phase contrast for investigation of microorganisms by optical microscopy used a neutral filter Zeiss NG 10/1 that could be controllably slid at a certain azimuthal angle below the aperture condenser diaphragm of the microscope phase contrast. Two ways of application are described depending on the type of the microscope: (1) in a special holder, and (2) fixed on a rubber ring. The device enabled us to obtain excellent results in the area of both optical microscopy and microphotography. With the microorganisms visualized, a better resolution, higher contrast and a significant 3D effect were obtained; outer morphology and organelles (chloroplasts, nuclei, granules, oil reserve vacuoles, etc.) could also be investigated.
一种新的方法提供了一个缓解相差对比为调查微生物的光学显微镜使用一个中性滤光片蔡司 NG 10/1 ，可以可控地滑在一定的方位角低于孔径聚光镜的显微镜相差。两种应用方式描述取决于显微镜的类型：（1）在一个特殊的支架，和（2）固定在橡胶环上。该装置使我们能够获得良好的效果，在光学显微镜和显微摄影领域。与微生物可视化，更好的分辨率，更高的对比度和显著的三维效果；外形态和细胞器（叶绿体，细胞核，颗粒，油储备液泡等）也可以进行调查。
