Laboratory of Molecular Structure Characterization, Institute of Microbiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
Folia Microbiol (Praha). 2012 Nov;57(6):509-12. doi: 10.1007/s12223-012-0162-1. Epub 2012 Jun 1.
Four simple sets for digital microphotography are described that have been tested with the Carl Zeiss Jena, Meopta Prague, Lambda Prague, and LOMO Sankt Petersburg microscopes and with DSLR Nikon D 70 and Nikon D 300 cameras. They permit precise image focusing in the camera using a prism Zeiss. The sets make use of commonly available extensions Zeiss, Praktica and reductions Nikon-Praktica manufactured by ROWI (without a lens) or HAMA (with a lens). An extension has further been designed and manufactured for connecting the DSLR Nikon D 300 camera fitted with the HAMA reduction (only with a lens) and a focusing extensible prism with Zeiss Jena light measurement. It permits a precise image focusing of low light intensity objects (autofluorescence or low-contrast or moving objects when using positive or negative phase contrast). The sets are applicable to all microscopes constructed according to German DIN industrial standards.
描述了四套用于数字显微摄影的简易装置,这些装置已在 Carl Zeiss Jena、Meopta Prague、Lambda Prague 和 LOMO Sankt Petersburg 显微镜以及 Nikon D 70 和 Nikon D 300 数码单反相机上进行了测试。它们允许使用 Zeiss 棱镜在相机中进行精确的图像对焦。这些套件利用了常见的 ROWI(无镜头)或 HAMA(带镜头)制造的 Zeiss、Praktica 扩展和 Nikon-Praktica 缩小。还进一步设计和制造了一个扩展,用于连接配备 HAMA 缩小(仅带镜头)和 Zeiss Jena 光测量可伸缩聚焦棱镜的 Nikon D 300 数码单反相机,以便对低光强度物体(自体荧光或低对比度或使用正相或负相差对比时的运动物体)进行精确的图像聚焦。这些套件适用于所有符合德国 DIN 工业标准制造的显微镜。