Nhiem Nguyen Xuan, Kim Ki Cheon, Kim Areum-Daseul, Hyun Jin Won, Kang Hee Kyoung, Van Kiem Phan, Van Minh Chau, Thu Vu Kim, Tai Bui Huu, Kim Jeong Ah, Kim Young Ho
College of Pharmacy, Chungnam National University, Daejeon, Korea.
J Asian Nat Prod Res. 2011 Jan;13(1):56-61. doi: 10.1080/10286020.2010.525743.
By various chromatographic methods, one new phenylpropanoid, acanthopanic acid (1), and three known compounds, 1,2-O-dicaffeoylcyclopenta-3-ol (2), (4S)-α-terpineol 8-O-β-D-glucopyranoside (3), and rutin (4), were isolated from the methanol extract of the Acanthopanax koreanum leaves. Their structures were elucidated on the basis of spectroscopic analyses, and their antioxidant activities were evaluated by the intracellular reactive oxygen species (ROS) radical scavenging 2',7'-dichlorofluorescein diacetate assay. Among them, compounds 1, 2, and 4 showed significant scavenging capacity with IC(50) values of 3.8, 2.6, and 2.9 μM, respectively, and compound 3 showed weak scavenging capacity with the inhibition rate of 37% at 40 μM.
通过各种色谱方法，从辽东楤木叶的甲醇提取物中分离出一种新的苯丙素类化合物刺五加酸（1）和三种已知化合物1,2 - O - 二咖啡酰环戊 - 3 - 醇（2）、（4S）-α - 松油醇8 - O - β - D - 吡喃葡萄糖苷（3）和芦丁（4）。基于光谱分析阐明了它们的结构，并通过细胞内活性氧（ROS）自由基清除2',7'-二氯荧光素二乙酸酯测定法评估了它们的抗氧化活性。其中，化合物1、2和4表现出显著的清除能力，IC(50)值分别为3.8、2.6和2.9 μM，化合物3表现出较弱的清除能力，在40 μM时抑制率为37%。
