Department of Clinical Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.
Muscle Nerve. 2011 Feb;43(2):157-63. doi: 10.1002/mus.21895. Epub 2010 Nov 16.
Adipose tissue is a good source for isolation of cells with stem-cell-like properties. The effects of undifferentiated cultured bone marrow stromal cells (BMSCs) and omental adipose-derived nucleated cells (OADNCs) on peripheral nerve regeneration were compared in a rat nerve regeneration model. A 10-mm sciatic nerve defect was bridged using a vein graft. In one group, the vein was filled with BMSCs and in the other group with OADNCs. Functional study, morphometric indices, and immunohistochemistry indicated there was no significant difference (P > 0.05) between groups in recovery of regenerated axons at 4, 8, and 12 weeks after surgery. OADNCs enhanced regeneration similar to undifferentiated BMSCs. These observations suggest OADNCs represent an effective and cost-saving cell population due to the shortened time interval from tissue collection to cell injection as well as procedural simplicity. This approach is clinically translatable toward new methods for enhanced peripheral nerve repair without the limitations of BMSC.
脂肪组织是分离具有干细胞样特性细胞的良好来源。在大鼠周围神经再生模型中，比较了未分化培养的骨髓基质细胞（BMSCs）和大网膜脂肪源性有核细胞（OADNCs）对周围神经再生的影响。使用静脉移植物桥接 10mm 坐骨神经缺损。在一组中，静脉内充满了 BMSCs，而在另一组中则充满了 OADNCs。功能研究、形态计量学和免疫组织化学结果表明，术后 4、8 和 12 周，再生轴突的恢复在两组之间没有显著差异（P>0.05）。OADNCs 增强了与未分化的 BMSCs 相似的再生。这些观察结果表明，由于从组织采集到细胞注射的时间间隔缩短以及操作简单，OADNCs 代表了一种有效且节省成本的细胞群体。这种方法可临床转化为增强周围神经修复的新方法，而没有 BMSC 的局限性。
