Department of Chemistry, Queen's University, Kingston, ON, Canada.
Electrophoresis. 2011 Jan;32(2):223-9. doi: 10.1002/elps.201000442. Epub 2010 Dec 17.
Joule heating, arising from the electric current passing through the capillary, causes many undesired effects in CE that ultimately result in band broadening. The use of narrow-bore capillaries helps to solve this problem as smaller cross-sectional area results in decreased Joule heating and the rate of heat dissipation is increased by the larger surface-to-volume ratio. Issues arising from such small capillaries, such as poor detection sensitivity, low loading capacity and high flow-induced backpressure (complicating capillary loading) can be avoided by using a bundle of small capillaries operating simultaneously that share buffer reservoirs. Microstructured fibres, originally designed as waveguides in the telecommunication industry, are essentially a bundle of parallel ∼5 μm id channels that extend the length of a fibre having otherwise similar dimensions to conventional CE capillaries. This work presents the use of microstructured fibres for CZE, taking advantage of their relatively high surface-to-volume ratio and the small individual size of each channel to effect highly efficient separations, particularly for dye-labelled peptides.
焦耳加热是由于电流通过毛细管引起的，它会在 CE 中产生许多不良影响，最终导致谱带展宽。使用细内径毛细管有助于解决这个问题，因为较小的截面积会降低焦耳加热，而较大的表面积与体积比会增加散热速率。小毛细管会出现一些问题，如检测灵敏度差、载样量低和流动引起的背压高（使毛细管进样复杂化），这些问题可以通过同时使用一组共享缓冲液储器的小毛细管来避免。微结构光纤最初是为电信行业的波导而设计的，它实质上是一束平行的约 5 μm 内径通道，这些通道延伸了光纤的长度，而光纤的其他尺寸与传统的 CE 毛细管相似。本工作利用微结构光纤相对较高的表面积与体积比以及每个通道的较小尺寸，展示了其在 CZE 中的应用，实现了高效的分离，特别是对染料标记的肽。
