Department of Chemistry, Selcuk University, Konya, Turkey.
J Sep Sci. 2011 Feb;34(4):393-401. doi: 10.1002/jssc.201000690. Epub 2011 Jan 20.
A new calix[4]crown-5 macrocycle-bonded silica stationary phase (CL-CIMS) was prepared and applied at the same time to develop a chromatographic procedure to separate aromatic amines, phenols and drugs in this study. The chromatographic behaviors of the prepared stationary phase for these analytes were studied and compared with those of ODS (octadecylsilane). The effect of organic modifier content and pH of the mobile phase on retention and selectivity of these compounds were investigated. Some aromatic amines, phenols or drugs on CL-CIMS were successfully separated. The results show that CL-CIMS exhibits high selectivities for the above analytes in high aqueous mobile phases and a bright prospect in routine, fast separation of aromatic amines, phenols and drug compounds. From chromatographic data, it can be concluded that hydrophobic interaction is mainly responsible for the retention behavior as well as hydrogen-bonding interaction, π-π and dipole-dipole interaction.
一种新型杯[4]冠-5 大环键合硅胶固定相（CL-CIMS）被制备出来，并同时应用于开发一种色谱程序，以分离本研究中的芳香胺、酚类和药物。研究了制备的固定相对这些分析物的色谱行为，并与 ODS（十八烷基硅烷）进行了比较。考察了流动相有机改性剂含量和 pH 值对这些化合物保留和选择性的影响。一些芳香胺、酚类或药物在 CL-CIMS 上得到了成功分离。结果表明，CL-CIMS 在高水相流动相中对上述分析物表现出高选择性，在常规、快速分离芳香胺、酚类和药物化合物方面具有广阔的前景。从色谱数据可以得出结论，疏水性相互作用是保留行为的主要原因，氢键相互作用、π-π 相互作用和偶极-偶极相互作用也是保留行为的主要原因。
