Spangenberg V E, Dadashev S Ia, Matveevskiĭ S N, Kolomiets O L, Bogdanov Iu F
Genetika. 2010 Oct;46(10):1363-6.
Fluorochrome-labeled oligonucleotides (n = 44) corresponding to mouse genome repetitive sequences were hybridized in situ with pachytene nuclei of mouse spermatocytes. Signals of the repetitive sequences MaLR, MER, and (GT)22 were found to be dispersed through chromatin, and signals of BI 1 repeats and minisatellites were mostly attached to synaptonemal complexes immunostained with anti-SYCP3 antibodies. These results suggest that B 1 repeats and minisatellites are candidates for sequences anchoring chromatin to synaptonemal complexes.
将与小鼠基因组重复序列对应的荧光染料标记寡核苷酸(n = 44)与小鼠精母细胞的粗线期细胞核进行原位杂交。发现重复序列MaLR、MER和(GT)22的信号分散在染色质中,而BI 1重复序列和微卫星的信号大多附着在用抗SYCP3抗体免疫染色的联会复合体上。这些结果表明,B 1重复序列和微卫星是将染色质锚定到联会复合体的序列候选物。