Pasteels B, Rogers J, Blachier F, Pochet R
Laboratoire d'Histologie, Faculté de Médecine, Université Libre de Bruxelles (ULB), Belgium.
Vis Neurosci. 1990 Jul;5(1):1-16. doi: 10.1017/s0952523800000031.
Calbindin-D28K and calretinin are homologous calcium-binding proteins localized in many neurons of the central nervous systems. We have compared polyclonal antibodies against calbindin and calretinin and have shown by western blots using purified calbindin and calretinin from rat that (1) anti-calretinin does not recognize calbindin and (2) anti-calbindin presents some cross-reactivity with calretinin. In this report, we have compared by immunohistochemistry the localization of both calcium-binding proteins in the retina of monkey, pig, sheep, rat, cat, pigeon, and salamander. These results are compared with previous data for chick. There are many differences between species and not within species, but some aspects of the distribution are conserved. All species, except rat and monkey, have some cones which contain calbindin only. Most species also have some bipolar cells containing calbindin only. Calretinin is rarely seen in photoreceptors or bipolar cells. All species have horizontal cells which contain calretinin or calbindin or both. All species have amacrine cells and ganglion cells containing one or other protein. In the cat ganglion cell layer, the calretinin antisera define a new, asymmetric, type of cell.
钙结合蛋白-D28K和钙视网膜蛋白是定位于中枢神经系统许多神经元中的同源钙结合蛋白。我们比较了抗钙结合蛋白和抗钙视网膜蛋白的多克隆抗体，并通过使用从大鼠中纯化的钙结合蛋白和钙视网膜蛋白进行的蛋白质印迹分析表明：（1）抗钙视网膜蛋白不能识别钙结合蛋白；（2）抗钙结合蛋白与钙视网膜蛋白存在一些交叉反应。在本报告中，我们通过免疫组织化学比较了钙结合蛋白在猴、猪、羊、大鼠、猫、鸽和蝾螈视网膜中的定位。这些结果与之前鸡的相关数据进行了比较。物种之间存在许多差异，而非物种内部，但分布的某些方面是保守的。除大鼠和猴外，所有物种都有一些仅含钙结合蛋白的视锥细胞。大多数物种也有一些仅含钙结合蛋白的双极细胞。钙视网膜蛋白在光感受器或双极细胞中很少见。所有物种都有含钙视网膜蛋白或钙结合蛋白或两者皆有的水平细胞。所有物种都有含其中一种蛋白的无长突细胞和神经节细胞。在猫的神经节细胞层中，抗钙视网膜蛋白血清鉴定出一种新的、不对称的细胞类型。
