Department of Medical Microbiology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.
Med Mycol. 2011 Jul;49(5):556-60. doi: 10.3109/13693786.2010.551424. Epub 2011 Jan 24.
This study was conducted to determine the proteinase, phospholipase, and biofilm forming abilities of Candida isolates in blood cultures of specimens from patients at the University Malaya Medical Center, Kuala Lumpur, Malaysia. Proteinase and phospholipase activities were detected in 93.7% and 73.3%, respectively, of 15 Candida albicans isolates. Amongst the 26 non-C. albicans Candida isolates, proteinase and phospholipase activities were detected in 88.5% and 7.7% of the isolates, respectively. There was no significant difference in the expression levels of proteinase amongst the Candida isolates studied (P = 0.272), but the phospholipase activity of C. albicans was significantly higher than that of the non-C. albicans Candida isolates (P = 0.003). There was no significant difference in the biofilm forming abilities of C. albicans and non-C. albicans Candida isolates on the polystyrene microtiter wells (P = 0.379). In addition, the findings of this study demonstrate increased resistance of Candida isolates in biofilms to amphotericin and fluconazole, as compared to their planktonic counterparts.
本研究旨在确定马来西亚吉隆坡马来亚大学医学中心患者标本血液培养物中分离出的念珠菌的蛋白酶、磷脂酶和生物膜形成能力。在 15 株白色念珠菌分离株中，分别检测到 93.7%和 73.3%具有蛋白酶和磷脂酶活性。在 26 株非白色念珠菌念珠菌分离株中，分别有 88.5%和 7.7%的分离株检测到蛋白酶和磷脂酶活性。在所研究的念珠菌分离株中，蛋白酶的表达水平没有显著差异（P = 0.272），但白色念珠菌的磷脂酶活性明显高于非白色念珠菌念珠菌分离株（P = 0.003）。在聚苯乙烯微量滴定孔中，白色念珠菌和非白色念珠菌念珠菌分离株的生物膜形成能力没有显著差异（P = 0.379）。此外，本研究的结果表明，与浮游细胞相比，生物膜中的念珠菌分离株对两性霉素和氟康唑的耐药性增加。
