Candidemia is often associated with high mortality, and and are common causes of this disease. The pathogenicity characteristics of specific spp. that cause candidemia in Thailand are poorly understood. This study aimed to characterize the virulence factors of spp. Thirty-eight isolates of different species from blood cultures were evaluated for their virulence properties, including exoenzyme and biofilm production, cell surface hydrophobicity, tissue invasion, epithelial cell damage, morphogenesis, and phagocytosis resistance; the identity and frequency of mutations in contributing to azole-resistance were also determined. had the highest epithelial cell invasion rate and phospholipase activity, with true hyphae formation, whereas produced the most biofilm, hydrophobicity, protease activity, and host cell damage and true hyphae formation. mutations Y132F and S154F were observed in all azole-resistant . had the most hemolytic activity while cell invasion was low with no morphologic transition. was more easily phagocytosed than other species. generated pseudohyphae but not hyphae and did not exhibit any trends in exoenzyme production. This knowledge will be crucial for understanding the pathogenicity of spp. and will help to explore antivirulence-based treatment.