通过透射电子显微镜观察神经酰胺通道。
Visualization of ceramide channels by transmission electron microscopy.
作者信息
Samanta Soumya, Stiban Johnny, Maugel Timothy K, Colombini Marco
机构信息
Department of Biology, University of Maryland, College Park, MD 20742, USA.
出版信息
Biochim Biophys Acta. 2011 Apr;1808(4):1196-201. doi: 10.1016/j.bbamem.2011.01.007. Epub 2011 Jan 19.
Abstract
Functional studies have shown that the sphingolipid ceramide, self-assembles in phospholipid membranes to form large channels capable of allowing proteins to cross the membrane. Here these channels are visualized by negative stain transmission electron microscopy. The images contain features consistent with stain-filled pores having a roughly circular profile. There is no indication of tilt, and the results are consistent with the formation of right cylinders. The sizes of the pores range from 5 to 40nm in diameter with an asymmetric distribution indicating no apparent upper size limit. The size distribution matches well with the distribution of sizes calculated from electrophysiological measurements.
摘要
功能研究表明，鞘脂神经酰胺在磷脂膜中自组装形成大通道，能够使蛋白质穿过膜。在这里，这些通道通过负染透射电子显微镜观察到。图像包含与大致呈圆形轮廓的充满染色剂的孔一致的特征。没有倾斜迹象，结果与直圆柱体的形成一致。孔的直径范围为5至40纳米，不对称分布表明没有明显的尺寸上限。尺寸分布与根据电生理测量计算出的尺寸分布非常匹配。
相似文献
1
Visualization of ceramide channels by transmission electron microscopy.
Biochim Biophys Acta. 2011 Apr;1808(4):1196-201. doi: 10.1016/j.bbamem.2011.01.007. Epub 2011 Jan 19.
2
Ceramide Channels.
Adv Exp Med Biol. 2019;1159:33-48. doi: 10.1007/978-3-030-21162-2_3.
3
Membrane channels formed by ceramide.
Handb Exp Pharmacol. 2013(215):109-26. doi: 10.1007/978-3-7091-1368-4_6.
4
The lipids C2- and C16-ceramide form large stable channels. Implications for apoptosis.
J Biol Chem. 2000 Dec 8;275(49):38640-4. doi: 10.1074/jbc.C000587200.
5
Ceramide channels and mitochondrial outer membrane permeability.
J Bioenerg Biomembr. 2017 Feb;49(1):57-64. doi: 10.1007/s10863-016-9646-z. Epub 2016 Jan 22.
6
Very long chain ceramides interfere with C16-ceramide-induced channel formation: A plausible mechanism for regulating the initiation of intrinsic apoptosis.
Biochim Biophys Acta. 2015 Feb;1848(2):561-7. doi: 10.1016/j.bbamem.2014.11.018. Epub 2014 Nov 21.
7
Ceramide channels: influence of molecular structure on channel formation in membranes.
Biochim Biophys Acta. 2012 May;1818(5):1291-301. doi: 10.1016/j.bbamem.2012.02.010. Epub 2012 Feb 15.
8
Ceramide binding to anandamide increases its half-life and potentiates its cytotoxicity in human neuroblastoma cells.
Chem Phys Lipids. 2017 Jun;205:11-17. doi: 10.1016/j.chemphyslip.2017.04.001. Epub 2017 Apr 4.
9
Asymmetric addition of ceramides but not dihydroceramides promotes transbilayer (flip-flop) lipid motion in membranes.
Biophys J. 2005 Jan;88(1):348-59. doi: 10.1529/biophysj.104.050690. Epub 2004 Oct 1.
10
Ceramide modulates the lipid membrane organization at molecular and supramolecular levels.
Chem Phys Lipids. 2003 Jan;122(1-2):147-52. doi: 10.1016/s0009-3084(02)00185-8.
引用本文的文献
1
Ceramide signaling in immunity: a molecular perspective.
Lipids Health Dis. 2025 Jul 1;24(1):225. doi: 10.1186/s12944-025-02642-2.
2
Purification and Characterization of Mitochondrial Mg-Independent Sphingomyelinase from Rat Brain.
Mol Cells. 2023 Sep 30;46(9):545-557. doi: 10.14348/molcells.2023.0074. Epub 2023 Jun 12.
3
Sphingolipid lysosomal storage diseases: from bench to bedside.
Lipids Health Dis. 2021 May 3;20(1):44. doi: 10.1186/s12944-021-01466-0.
4
A Novel Proteomic Method Reveals NLS Tagging of T-DM1 Contravenes Classical Nuclear Transport in a Model of HER2-Positive Breast Cancer.
Mol Ther Methods Clin Dev. 2020 Sep 1;19:99-119. doi: 10.1016/j.omtm.2020.08.016. eCollection 2020 Dec 11.
5
Improving Receptor-Mediated Intracellular Access and Accumulation of Antibody Therapeutics-The Tale of HER2.
Antibodies (Basel). 2020 Jul 13;9(3):32. doi: 10.3390/antib9030032.
6
Body Mass Index in Multiple Sclerosis modulates ceramide-induced DNA methylation and disease course.
EBioMedicine. 2019 May;43:392-410. doi: 10.1016/j.ebiom.2019.03.087. Epub 2019 Apr 10.
7
Advances in determining signaling mechanisms of ceramide and role in disease.
J Lipid Res. 2019 May;60(5):913-918. doi: 10.1194/jlr.S092874. Epub 2019 Mar 7.
8
Ceramide Metabolism Balance, a Multifaceted Factor in Critical Steps of Breast Cancer Development.
Int J Mol Sci. 2018 Aug 26;19(9):2527. doi: 10.3390/ijms19092527.
9
Lipotoxicty in yeast: a focus on plasma membrane signalling and membrane contact sites.
FEMS Yeast Res. 2018 Jun 1;18(4). doi: 10.1093/femsyr/foy034.
10
Visualization of ceramide channels in lysosomes following endogenous palmitoyl-ceramide accumulation as an initial step in the induction of necrosis.
Biochem Biophys Rep. 2017 Mar 13;11:174-181. doi: 10.1016/j.bbrep.2017.02.010. eCollection 2017 Sep.
本文引用的文献
1
Regulation of ceramide channels by Bcl-2 family proteins.
FEBS Lett. 2010 May 17;584(10):2128-34. doi: 10.1016/j.febslet.2010.02.032. Epub 2010 Feb 14.
2
Ceramide and activated Bax act synergistically to permeabilize the mitochondrial outer membrane.
Apoptosis. 2010 May;15(5):553-62. doi: 10.1007/s10495-009-0449-0.
3
Undulating tubular liposomes through incorporation of a synthetic skin ceramide into phospholipid bilayers.
Langmuir. 2009 Sep 15;25(18):10422-5. doi: 10.1021/la9010899.
4
Diversity and complexity of ceramide generation after exposure of jurkat leukemia cells to irradiation.
Int J Radiat Oncol Biol Phys. 2009 Mar 15;73(4):1211-8. doi: 10.1016/j.ijrobp.2008.11.033.
5
Principles of bioactive lipid signalling: lessons from sphingolipids.
Nat Rev Mol Cell Biol. 2008 Feb;9(2):139-50. doi: 10.1038/nrm2329.
6
Anti-apoptotic Bcl-2 Family Proteins Disassemble Ceramide Channels.
J Biol Chem. 2008 Mar 14;283(11):6622-30. doi: 10.1074/jbc.M706115200. Epub 2008 Jan 2.
7
Ceramide forms channels in mitochondrial outer membranes at physiologically relevant concentrations.
Mitochondrion. 2006 Jun;6(3):118-25. doi: 10.1016/j.mito.2006.03.002. Epub 2006 Mar 29.
8
Dihydroceramide hinders ceramide channel formation: Implications on apoptosis.
Apoptosis. 2006 May;11(5):773-80. doi: 10.1007/s10495-006-5882-8.
9
Searching for the molecular arrangement of transmembrane ceramide channels.
Biophys J. 2006 Apr 1;90(7):2414-26. doi: 10.1529/biophysj.105.071977. Epub 2006 Jan 13.
10
A mitochondrial pool of sphingomyelin is involved in TNFalpha-induced Bax translocation to mitochondria.
Biochem J. 2005 Mar 15;386(Pt 3):445-51. doi: 10.1042/BJ20041627.
Zotero 插件