Department of Chemistry, University of Louisville, Louisville, KY 40292, USA.
Biomaterials. 2011 Apr;32(10):2683-8. doi: 10.1016/j.biomaterials.2010.12.047. Epub 2011 Jan 20.
Functionalization of iron oxide nanoparticles with quaternary ammonium ion-based aminooxy and oxime ether substrates provides a flexible route for generating magnetic gene delivery vectors. Using the MCF-7 breast cancer cell line, our findings show that pDNA magnetoplexes derived from the lipid-coated nanoparticle formulation dMLP transfect in the presence of 10% serum with or without magnetic assistance at significantly higher levels than a commonly used cationic liposome formulation, based on luciferase assay. The present ion-pairing, click chemistry approach furnishes Fe(3)O(4) nanoparticles with lipid layers. The resultant magnetic nanovectors serve as transfection enhancers for otherwise transfection-inactive materials.
用季铵离子基氨氧基和肟醚基质对氧化铁纳米粒子进行功能化，为生成磁性基因传递载体提供了一条灵活的途径。利用 MCF-7 乳腺癌细胞系，我们的研究结果表明，源自脂质包覆纳米粒子制剂 dMLP 的 pDNA 磁复合物在 10%血清存在或不存在磁辅助的情况下进行转染，其转染效率比常用的阳离子脂质体制剂高得多，基于荧光素酶检测。本研究中的离子配对、点击化学方法为 Fe(3)O(4)纳米粒子提供了脂质层。所得磁性纳米载体可用作转染非活性材料的转染增强剂。
