Tsou K C, Lo K W, Herberman R B
J Natl Cancer Inst. 1978 Oct;61(4):1077-83.
To provide information on the role of nucleases in oncogenic virus infection, the activities of 3'-nucleotide phosphodiesterase (3'-NPDase), 5'-nucleotide phosphodiesterase (5'-NPDase), acid deoxyribonuclease (DNase II), and 3',5'-cyclic AMP phosphodiesterase (cAMPDase) in spleen extracts of murine sarcoma virus-infected C57BL/6 inbred mice were studied. At the peak of tumor growth and of the cell-mediated cytotoxic response (CMC) against tumor-associated antigens, 3'-NPDase, 5'-NPDase, and DNase II all showed depressed activities in the spleen, whereas the activity of cAMPDase in the spleen increased at the peak of CMC and remained elevated thereafter. Serum enzyme activities of the infected mice were also determined, and only 3'-NPD-ase in serum correlated well with CMC. Inasmuch as the correlation of the tumor growth with CMC was established in this system, further study on tumors with variance between CMC and growth is necessary to determine if serum 3'-NPDase is a useful biochemical marker for CMC in vivo.
为了提供核酸酶在致癌病毒感染中的作用信息，对感染小鼠肉瘤病毒的C57BL/6近交系小鼠脾脏提取物中的3'-核苷酸磷酸二酯酶（3'-NPDase）、5'-核苷酸磷酸二酯酶（5'-NPDase）、酸性脱氧核糖核酸酶（DNase II）和3',5'-环磷酸腺苷磷酸二酯酶（cAMPDase）的活性进行了研究。在肿瘤生长高峰期以及针对肿瘤相关抗原的细胞介导细胞毒性反应（CMC）高峰期，脾脏中的3'-NPDase、5'-NPDase和DNase II活性均降低，而脾脏中cAMPDase的活性在CMC高峰期升高，并在随后保持升高。还测定了感染小鼠的血清酶活性，血清中只有3'-NPDase与CMC密切相关。鉴于该系统中已确定肿瘤生长与CMC的相关性，有必要对CMC和生长存在差异的肿瘤进行进一步研究，以确定血清3'-NPDase是否是体内CMC的有用生化标志物。
