Filice G, Soldini L, Orsolini P, Razzini E, Chiapparoli L, Gulminetti R, Cattaneo E, Achilli G
IRCCS Policlinico S. Matteo, Department of Infectious Diseases, University of Pavia.
Microbiologica. 1991 Jan;14(1):1-7.
We have developed a system consisting of two separate ELISA, one designed to detect antibodies to HIV gag gene (p24) and the other to detect antibodies to HIV env gene (gp41). The antigen used in these ELISA was produced as recombinant DNA-derived proteins expressed in E. coli for HIV gag gene (p24) and synthetic peptide for the HIV env gene (gp41). These HIV (env-gag) ELISA, that provide independent determinations of the antibody response to the core and envelope proteins, are highly specific and sensitive. In this work we have demonstrated that determinations of antibodies such as those to p24 and gp41 by HIV (env-gag) ELISA are among the criteria for a confirmation procedure, and sensitivity one (gp41) and/or both these determination should be equal or greater than the sensitivity of W.B. In addition, the procedure should be objective and standardized and the antigen source used should be different from that adopted in the "classical" W.B. and screening test. In view of these considerations, this HIV (env-gag) ELISA could be used as a reliable alternative to W.B. for confirmation of antibody detection.
我们开发了一种由两种独立酶联免疫吸附测定（ELISA）组成的系统，一种用于检测针对HIV gag基因（p24）的抗体，另一种用于检测针对HIV env基因（gp41）的抗体。这些ELISA中使用的抗原，对于HIV gag基因（p24）是作为在大肠杆菌中表达的重组DNA衍生蛋白产生的，对于HIV env基因（gp41）是合成肽。这些HIV（env - gag）ELISA能够独立测定针对核心蛋白和包膜蛋白的抗体反应，具有高度特异性和敏感性。在这项工作中，我们已经证明，通过HIV（env - gag）ELISA测定诸如针对p24和gp41的抗体是确认程序的标准之一，并且敏感性之一（gp41）和/或这两种测定都应等于或大于免疫印迹法（W.B.）的敏感性。此外，该程序应客观且标准化，所使用的抗原来源应与“经典”W.B.和筛查试验中采用的不同。鉴于这些考虑因素，这种HIV（env - gag）ELISA可作为W.B.的可靠替代方法用于抗体检测的确认。
