Department of Genetics, University of North Carolina, 115 Mason Farm Rd., CB 7250, Chapel Hill, NC 27599, USA.
Dev Biol. 2011 Apr 1;352(1):40-7. doi: 10.1016/j.ydbio.2011.01.015. Epub 2011 Jan 21.
The HMG-Box transcription factor SOX2 is expressed in neural progenitor populations throughout the developing and adult central nervous system and is necessary to maintain their progenitor identity. However, it is unclear whether SOX2 levels are uniformly expressed across all neural progenitor populations. In the developing dorsal telencephalon, two distinct populations of neural progenitors, radial glia and intermediate progenitor cells, are responsible for generating a majority of excitatory neurons found in the adult neocortex. Here we demonstrate, using both cellular and molecular analyses, that SOX2 is differentially expressed between radial glial and intermediate progenitor populations. Moreover, utilizing a SOX2(EGFP) mouse line, we show that this differential expression can be used to prospectively isolate distinct, viable populations of radial glia and intermediate cells for in vitro analysis. Given the limited repertoire of cell-surface markers currently available for neural progenitor cells, this provides an invaluable tool for prospectively identifying and isolating distinct classes of neural progenitor cells from the central nervous system.
HMG-Box 转录因子 SOX2 表达于整个中枢神经系统发育和成年期的神经祖细胞群体中，对维持其祖细胞特性是必需的。然而，SOX2 水平是否在所有神经祖细胞群体中均匀表达尚不清楚。在发育中的背侧端脑，两种不同的神经祖细胞群体，放射状胶质细胞和中间祖细胞，负责产生成年新皮质中发现的大多数兴奋性神经元。在这里，我们使用细胞和分子分析证明，SOX2 在放射状胶质细胞和中间祖细胞群体之间存在差异表达。此外，利用 SOX2(EGFP) 小鼠品系，我们表明这种差异表达可用于前瞻性分离不同的、有活力的放射状胶质细胞和中间细胞群体进行体外分析。鉴于目前用于神经祖细胞的细胞表面标志物的有限组合，这为从中枢神经系统中前瞻性鉴定和分离不同类型的神经祖细胞提供了宝贵的工具。
