Rogers Nicholas, Cheah Pike-See, Szarek Eva, Banerjee Kakoli, Schwartz Jeffrey, Thomas Paul
Discipline of Biochemistry, School of Molecular and Biomedical Sciences, University of Adelaide, Adelaide, Australia.
Gene Expr Patterns. 2013 Oct;13(7):240-8. doi: 10.1016/j.gep.2013.04.004. Epub 2013 May 9.
Previous studies have shown that Sox3 is expressed in nascent neuroprogenitor cells and is functionally required in mammals for development of the dorsal telencephalon and hypothalamus. However, Sox3 expression during embryonic and adult neurogenesis has not been examined in detail. Using a SOX3-specific antibody, we show that murine SOX3 expression is maintained throughout telencephalic neurogenesis and is restricted to progenitor cells with neuroepithelial and radial glial morphologies. We also demonstrate that SOX3 is expressed within the adult neurogenic regions and is coexpressed extensively with the neural stem cell marker SOX2 indicating that it is a lifelong marker of neuroprogenitor cells. In contrast to the telencephalon, Sox3 expression within the developing hypothalamus is upregulated in developing neurons and is maintained in a subset of differentiated hypothalamic cells through to adulthood. Together, these data show that Sox3 regulation is region-specific, consistent with it playing distinct biological roles in the dorsal telencephalon and hypothalamus.
先前的研究表明，Sox3在新生神经祖细胞中表达，在哺乳动物中，它是背侧端脑和下丘脑发育所必需的功能蛋白。然而，尚未对胚胎期和成体神经发生过程中Sox3的表达进行详细研究。我们使用SOX3特异性抗体，发现小鼠SOX3的表达在整个端脑神经发生过程中持续存在，并且仅限于具有神经上皮和放射状胶质细胞形态的祖细胞。我们还证明，SOX3在成体神经发生区域表达，并与神经干细胞标志物SOX2广泛共表达，这表明它是神经祖细胞的终生标志物。与端脑不同，发育中的下丘脑内的Sox3表达在发育中的神经元中上调，并在一部分分化的下丘脑细胞中持续表达直至成年。这些数据共同表明，Sox3的调控具有区域特异性，这与它在背侧端脑和下丘脑中发挥不同的生物学作用相一致。
