一种可靠的保存长尾噬菌体的方法。
A reliable method for storage of tailed phages.
机构信息
Department of Molecular Biology, University of Gdańsk, Kładki 24, 80-822 Gdańsk, Poland.
出版信息
J Microbiol Methods. 2011 Mar;84(3):486-9. doi: 10.1016/j.mimet.2011.01.007. Epub 2011 Jan 21.
PMID:21256885
Abstract
A universal and effective method for long-term storage of bacteriophages has not yet been described. We show that randomly selected tailed phages could be stored inside the infected cells at -80°C without a major loss of phage and host viability. Our results suggest the suitability of this method as a standard for phage preservation.
摘要
尚未描述一种通用且有效的噬菌体长期储存方法。我们表明，随机选择的尾部噬菌体可以在-80°C 的温度下储存在感染细胞内，而不会导致噬菌体和宿主活力的大量损失。我们的结果表明，该方法适合作为噬菌体保存的标准。
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