Wdowiak Mateusz, Paczesny Jan, Raza Sada
Institute of Physical Chemistry, Polish Academy of Sciences, Kasprzaka 44/52, 01-224 Warsaw, Poland.
Pharmaceutics. 2022 Sep 13;14(9):1936. doi: 10.3390/pharmaceutics14091936.
Phages are efficient in diagnosing, treating, and preventing various diseases, and as sensing elements in biosensors. Phage display alone has gained attention over the past decade, especially in pharmaceuticals. Bacteriophages have also found importance in research aiming to fight viruses and in the consequent formulation of antiviral agents and vaccines. All these applications require control over the stability of virions. Phages are considered resistant to various harsh conditions. However, stability-determining parameters are usually the only additional factors in phage-related applications. Phages face instability and activity loss when preserved for extended periods. Sudden environmental changes, including exposure to UV light, temperature, pH, and salt concentration, also lead to a phage titer fall. This review describes various formulations that impart stability to phage stocks, mainly focusing on polymer-based stabilization, encapsulation, lyophilization, and nano-assisted solutions.
噬菌体在诊断、治疗和预防各种疾病以及作为生物传感器中的传感元件方面都很有效。仅噬菌体展示在过去十年中就受到了关注，尤其是在制药领域。噬菌体在旨在对抗病毒的研究以及随后抗病毒药物和疫苗的研发中也具有重要意义。所有这些应用都需要控制病毒粒子的稳定性。噬菌体被认为对各种恶劣条件具有抗性。然而，稳定性决定参数通常是噬菌体相关应用中唯一的附加因素。长时间保存时，噬菌体会面临不稳定性和活性丧失。突然的环境变化，包括暴露于紫外线、温度、pH值和盐浓度，也会导致噬菌体滴度下降。本综述描述了赋予噬菌体悬液稳定性的各种制剂，主要侧重于基于聚合物的稳定化、封装、冻干和纳米辅助溶液。
