Vojteková H, Svorc J, Miertus S
Institute of Food Research, Bratislava, Czechoslovakia.
Neoplasma. 1990;37(6):667-74.
Mechanism of action of cis-diamminedichloroplatinum (II) (cis-DDP) was studied using genetically manipulated bacteria Escherichia coli K-12, recombinant PQ 37 (so-called SOS chromotest). Basic characteristics, namely strength of cis-DDP genotoxicity, influence of chloride ions on genotoxicity and dependence of genotoxicity versus time of cis-DDP aquation reaction were tested. The rate constant has been found to be 0.91 +/- 0.03 x 10(-4)s-1. Possibilities of the use of SOS chromotest in the determination of cis-DDP were found. The detection limit has been 0.09 mg/l in water as well as in urine. The amount of cis-DDP was determined in urine of patients after drug treatment. Analysis of clinical samples have been completed without any preseparation.
利用基因工程改造的大肠杆菌K-12重组体PQ 37（即所谓的SOS显色试验）研究了顺二氯二氨铂（II）（顺铂）的作用机制。测试了顺铂遗传毒性的基本特征，即氯离子对遗传毒性的影响以及遗传毒性与顺铂水合反应时间的关系。发现速率常数为0.91±0.03×10⁻⁴s⁻¹。发现了SOS显色试验用于测定顺铂的可能性。在水和尿液中的检测限均为0.09 mg/l。测定了药物治疗后患者尿液中的顺铂含量。临床样本分析在未进行任何预分离的情况下完成。
