Lei X F, Xing Q
Beijing Medical University.
Zhonghua Yu Fang Yi Xue Za Zhi. 1993 May;27(3):141-3.
Samples of variously-sized total suspended particles in the air of one sampling site of TaiYuan city were collected. The samples were extracted with acid and a simulated lung fluid (SLF) respectively. Both extracts mainly consisted of metallic elements. Using SOS chromotest as a means to test the gentoxity of the extracts and five metallic compounds, namely Cr6+, Ni2+, Pb2+, Mn2+ and Cd2+ were tested first. All the compounds tested could induce the SOS response to various extent, showing that the method was sensitive to metallic compounds. For the extracts of air particles, both the extracts of acid and of SLF of the smaller-sized particles could induce SOS response. This indicated the existence of metaklic genotoxicants in the smaller-sized particles. Being convenient to use fast and precise, the SOS Chromotest has its unique advantage for detecting carcinogenic metallic compounds.
采集了太原市某一采样点空气中各种大小的总悬浮颗粒物样本。分别用酸和模拟肺液（SLF）对样本进行提取。两种提取物主要由金属元素组成。以SOS显色试验作为检测提取物遗传毒性的方法，首先对铬（Ⅵ）、镍（Ⅱ）、铅（Ⅱ）、锰（Ⅱ）和镉（Ⅱ）这五种金属化合物进行了测试。所有测试的化合物都能在不同程度上诱导SOS反应，表明该方法对金属化合物敏感。对于空气颗粒物提取物，较小尺寸颗粒物的酸提取物和SLF提取物都能诱导SOS反应。这表明较小尺寸颗粒物中存在金属遗传毒物。SOS显色试验使用方便、快速且精确，在检测致癌金属化合物方面具有独特优势。
