Universidade de Franca, Avenida Dr Armando Salles de Oliveira, 201 Parque Universitário, 14404-600, Franca, São Paulo, Brazil.
J Appl Toxicol. 2011 Nov;31(8):714-9. doi: 10.1002/jat.1614. Epub 2011 Jan 24.
Artepillin C (3,5-diprenyl-p-coumaric acid), a major compound found in Brazilian green propolis and Baccharis dracunculifolia, shows anti-inflammatory, antibacterial, antiviral, antioxidant and antitumoral activities, among others. The aim of this study was to evaluate the genotoxic potential of artepillin C and its ability to prevent the chemically induced chromosome breakage or loss and the primary DNA damage using the micronucleus and comet assays in male Swiss mice, respectively. The animals were treated by gavage with different doses of artepillin C (0.4, 0.8 and 1.6 mg kg(-1) b.w.). For the antigenotoxicity assays, the different doses of artepillin C were administered simultaneously to doxorubicin (DXR; micronucleus test; 15 mg kg(-1) b.w.) and to methyl methanesulfonate (MMS; comet assay; 40 mg kg(-1) b.w.). The results showed that artepillin C itself was not genotoxic in the mouse micronucleus and comet assays. In the animals treated with artepillin C and DXR, the number of micronucleated reticulocytes was significantly lower in comparison with the animals treated only with DXR. Regarding antigenotoxicity, artepillin C at the tested doses significantly reduced the extent of DNA damage in liver cells induced by MMS.
阿魏酸 3,5-二烯丙基酯（Arttepillin C）是巴西绿蜂胶和 Baccharis dracunculifolia 中发现的主要化合物，具有抗炎、抗菌、抗病毒、抗氧化和抗肿瘤等活性。本研究旨在评估阿魏酸 3,5-二烯丙基酯的遗传毒性潜力及其在雄性瑞士小鼠中分别通过微核和彗星试验预防化学诱导的染色体断裂或丢失和原发性 DNA 损伤的能力。动物通过灌胃给予不同剂量的阿魏酸 3,5-二烯丙基酯（0.4、0.8 和 1.6mg/kg 体重）。对于抗原毒性试验，同时给予不同剂量的阿魏酸 3,5-二烯丙基酯与多柔比星（微核试验；15mg/kg 体重）和甲磺酸甲酯（彗星试验；40mg/kg 体重）。结果表明，阿魏酸 3,5-二烯丙基酯本身在小鼠微核和彗星试验中没有遗传毒性。在用阿魏酸 3,5-二烯丙基酯和多柔比星处理的动物中，与仅用多柔比星处理的动物相比，网织红细胞的微核数显著降低。关于抗原毒性，在测试剂量下，阿魏酸 3,5-二烯丙基酯可显著降低 MMS 诱导的肝细胞 DNA 损伤程度。
