a Universidade de Franca , Franca , São Paulo , Brazil.
Nutr Cancer. 2013;65(7):1098-103. doi: 10.1080/01635581.2013.815233. Epub 2013 Aug 5.
Artepillin C (3,5-diprenyl-p-coumaric acid) is one of the major phenolic compounds found in Brazilian green propolis, as well as in its botanical source, Baccharis dracunculifolia DC (Asteraceae). The present study evaluated the possible genotoxic and protective activities of artepillin C, in vitro, using methyl methanesulfonate (MMS) as a positive control, by comet and micronucleus assays. The cultures of Chinese hamster lung fibroblasts (V79 cells) were treated with different concentrations of artepillin C (2.5, 5.0, 10.0, and 20 μM). In antigenotoxicity assessment, the 3 concentrations of artepillin C (2.5, 5.0, and 10.0 μM) were associated with MMS (200 μM-comet assay and 400 μM-micronucleus assay). A statistically significant increase in the DNA damage and micronucleus frequencies was observed in the culture treated with the highest concentration of the artepillin C in comparison to the control group. All concentrations of artepillin C showed protective activity in relation to MMS-induced genotoxicity, which may be due to its antioxidant properties.
阿魏酸 3,5-二烯丙酯（3,5-二烯丙基对香豆酸）是巴西绿蜂胶以及其植物来源（菊科植物 Baccharis dracunculifolia DC）中主要的酚类化合物之一。本研究采用甲基甲磺酸（MMS）作为阳性对照，通过彗星试验和微核试验，评估了阿魏酸 3,5-二烯丙酯的潜在遗传毒性和保护活性。用不同浓度的阿魏酸 3,5-二烯丙酯（2.5、5.0、10.0 和 20 μM）处理中国仓鼠肺成纤维细胞（V79 细胞）的培养物。在抗原毒性评估中，阿魏酸 3,5-二烯丙酯（2.5、5.0 和 10.0 μM）的 3 个浓度与 MMS（彗星试验 200 μM 和微核试验 400 μM）相关联。与对照组相比，用最高浓度的阿魏酸 3,5-二烯丙酯处理的培养物中观察到 DNA 损伤和微核频率明显增加。所有浓度的阿魏酸 3,5-二烯丙酯对 MMS 诱导的遗传毒性均表现出保护活性，这可能与其抗氧化特性有关。
