Lavrent'eva E V, Dunaevskiĭ Ia E, Kozyreva L P, Radnagurueva A A, Namsaraev B B
Prikl Biokhim Mikrobiol. 2010 Nov-Dec;46(6):630-6.
Influence of nitrogen source on proteinases synthesis in aerobic alkalotolerant and halotolerant bacteria from soda-salt lakes of Transbaikalia was studied. Maximal accumulation of proteinases was revealed on medium with peptones. Introduction of various sources of nitrogen in the medium did not result in increase of enzyme activity in cultural liquid. It was indicated that secreting proteinases of the studied bacteria strains possess narrow substrate specificity, hydrolyze proteins and n-nitroanilide substrates have maximal activity during GlpAALpNA hydrolysis. Data of inhibitory analysis and substrate specificity of studied extracellular enzymes indicate that they belong to a class of serine proteinases of subtilisin-like type.
研究了氮源对来自外贝加尔地区苏打盐湖的需氧耐碱和耐盐细菌中蛋白酶合成的影响。在含有蛋白胨的培养基上发现蛋白酶积累量最大。向培养基中引入各种氮源并未导致培养液中酶活性增加。结果表明，所研究细菌菌株分泌的蛋白酶具有狭窄的底物特异性，可水解蛋白质，并且在水解GlpAALpNA时，对n-硝基苯胺底物具有最大活性。所研究的细胞外酶的抑制分析数据和底物特异性表明，它们属于枯草杆菌蛋白酶样丝氨酸蛋白酶类。
