Kallistova A Iu, Kevbrina M V, Pimenov N V, Rusanov I I, Rogozin D Iu, Wehrli B, Nozhevnikova A N
Mikrobiologiia. 2006 Nov-Dec;75(6):828-35.
The biogeochemical and molecular biological study of the chemocline and sediments of saline meromictic lakes Shira and Shunet (Khakass Republic, Russia) was performed. A marked increase in the rates of sulfate reduction and methanogenesis was revealed at the medium depths of the chemocline. The rates of these processes in the bottom sediments decreased with depth. The numbers of Bacteria, Archaea, and of sulfate-reducing bacteria (SRB) were determined by fluorescence in situ hybridization with rRNA specific oligonucleotide probes labeled with horseradish peroxidase and subsequent tyramide signal amplification. In the chemocline, both the total microbial numbers and those of Bacteria were shown to increase with depth. The archaea and SRB were present in almost equal numbers. In the lake sediments, a drastic decrease in microbial numbers with depth was revealed. SRB were found to prevail in the upper sediment layer and archaea in the lower one. This finding correlates with the measured rates of sulfate reduction and methanogenesis.
对俄罗斯哈卡斯共和国希拉湖和舒涅特湖这两个咸水半混合湖的化学跃层及沉积物进行了生物地球化学和分子生物学研究。在化学跃层的中等深度处，硫酸盐还原和甲烷生成速率显著增加。底部沉积物中这些过程的速率随深度降低。通过用辣根过氧化物酶标记的rRNA特异性寡核苷酸探针进行荧光原位杂交及随后的酪胺信号放大，测定了细菌、古菌和硫酸盐还原菌（SRB）的数量。在化学跃层中，微生物总数和细菌数量均随深度增加。古菌和SRB的数量几乎相等。在湖泊沉积物中，发现微生物数量随深度急剧减少。SRB在上层沉积物中占优势，古菌在下层沉积物中占优势。这一发现与测得的硫酸盐还原和甲烷生成速率相关。
