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红曲 NTU 568 中的安卡黄素和单菲酮 A 的抗肿瘤和抗炎特性。

Anti-tumor and anti-inflammatory properties of ankaflavin and monaphilone A from monascus purpureus NTU 568.

机构信息

Department of Biochemical Science and Technology, College of Life Science, National Taiwan University , Taipei 106, Taiwan, Republic of China.

出版信息

J Agric Food Chem. 2011 Feb 23;59(4):1124-30. doi: 10.1021/jf103652n. Epub 2011 Jan 24.

Abstract

An azaphilonidal derivative monaphilone A (MA) was recently isolated from the fermented products of Monascus purpureus NTU 568 by our laboratory. We report here the exploration of apoptosis-related and anti-inflammatory properties of MA and ankaflavin (AK) by some experiments about inducing death of human laryngeal carcinoma cell line HEp-2 and reducing inflammatory responses on murine macrophage RAW 264.7 cells. We employed a ssDNA enzyme-linked immunosorbent assay (ELISA) kit to investigate the nuclear changes of early apoptosis induced by AK and MA on HEp-2 cells and used a western blot and an enzyme activity assay to demonstrate the activation of caspase-3, caspase-8, and caspase-9 by MA and AK. Our studies revealed that AK and MA may decrease lipopolysaccharide (LPS)-induced inflammatory responses, including nitrite productions and expressions of inducible nitric oxide synthase (iNOS) and cyclooxygenase 2 (COX-2) in RAW 264.7 cells. All evidence support that azaphilonidal derivatives from M. purpureus NTU 568, such as AK and MA, are suitable for the development of chemotherapy or chemopreventive agents.

摘要

我们实验室最近从红曲 NTU568 的发酵产物中分离到一种氮杂菲并衍生物单甲菲酮 A(MA)。本文报告了 MA 和安卡黄素(AK)通过诱导人喉癌细胞系 HEp-2 死亡和降低鼠巨噬细胞 RAW264.7 细胞炎症反应,对细胞凋亡和抗炎作用的研究。我们采用 ssDNA 酶联免疫吸附测定(ELISA)试剂盒研究 AK 和 MA 对 HEp-2 细胞早期凋亡诱导的核变化,并采用 Western blot 和酶活性测定来证明 MA 和 AK 对 caspase-3、caspase-8 和 caspase-9 的激活作用。研究表明,AK 和 MA 可能降低脂多糖(LPS)诱导的 RAW264.7 细胞中一氧化氮合酶(iNOS)和环氧化酶 2(COX-2)的诱导表达和亚硝酸盐的产生,从而抑制炎症反应。所有证据表明,红曲 NTU568 中的氮杂菲并衍生物如 AK 和 MA 可用于开发化疗或化学预防药物。

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