Prathiba Subramanian, Sabareesh Varatharajan, Anbalagan Moorthy, Jayaraman Gurunathan
Department of Biotechnology, School of Bio Science and Technology, Vellore Institute of Technology, Vellore, 632014 Tamil Nadu India.
Advanced Centre for Bio Separation Technology, Vellore Institute of Technology, Vellore, 632014 Tamil Nadu India.
3 Biotech. 2021 Jun;11(6):276. doi: 10.1007/s13205-021-02724-9. Epub 2021 May 18.
The present study was aimed at evaluating the cytotoxic potential of selected halophilic bacterial metabolites. The use of the metabolomics approach in identifying the unexplored bioactive metabolites from halophilic bacterial isolate reduces time and complex experiments. In our study, we used UV/Visible spectroscopy, LC-MS/MS, and NMR to identify the metabolites present in the methanolic extract of the halophilic bacterium VITPS16. MTT assay revealed that metabolite fractions (S1-79.61% and S2-85.74%) possess cytotoxic activity. Colonogenic assay confirmed the cytotoxic potential of the fractions and apoptosis assays showed that 83.37% of the cells undergo apoptosis at 10 mg/mL concentration (MF-S2). The DNA binding studies revealed the metabolite fraction interacts with DNA resulting in cytotoxicity. The study states that MF- S2 induced an antiproliferative effect that led to apoptosis through DNA binding as one of the possible pathways. The toxicity analysis using zebrafish indicated that the metabolite fractions are non-toxic even at 10 mg/mL concentration. Fraction MF-S2 is found to contain phosphoethanolamines, glycerophospholipids, sphingolipids, apocarotenoid, enigmol and its analogue, ankaflavin and flavonoid type of metabolites, which have been previously reported to have anti-cancer activity.
The online version contains supplementary material available at 10.1007/s13205-021-02724-9.
本研究旨在评估所选嗜盐细菌代谢产物的细胞毒性潜力。使用代谢组学方法来鉴定嗜盐细菌分离株中未被探索的生物活性代谢产物，可减少时间和复杂的实验。在我们的研究中，我们使用紫外/可见光谱、液相色谱-串联质谱和核磁共振来鉴定嗜盐细菌VITPS16甲醇提取物中存在的代谢产物。MTT分析表明，代谢产物组分（S1 - 79.61%和S2 - 85.74%）具有细胞毒性活性。集落形成分析证实了这些组分的细胞毒性潜力，凋亡分析表明，在10 mg/mL浓度（MF - S2）下，83.37%的细胞发生凋亡。DNA结合研究表明，代谢产物组分与DNA相互作用导致细胞毒性。该研究指出，MF - S2诱导了抗增殖作用，通过与DNA结合作为可能的途径之一导致凋亡。使用斑马鱼的毒性分析表明，即使在10 mg/mL浓度下，代谢产物组分也无毒。发现组分MF - S2含有磷酸乙醇胺、甘油磷脂、鞘脂、脱辅基类胡萝卜素、enigmol及其类似物、ankaflavin和黄酮类代谢产物，这些代谢产物先前已被报道具有抗癌活性。
在线版本包含可在10.1007/s13205 - 021 - 02724 - 9获取的补充材料。
