Clinical characteristics of TIMP2, MMP2, and MMP9 gene polymorphisms in colorectal cancer.

BACKGROUND AND AIM

Genetic variations and the expression profile of matrix metalloproteinases (MMP) and tissue inhibitors of metalloproteinases (TIMP) are involved in the invasion and metastasis of colorectal cancer.

METHODS

The gene profiles of TIMP2 and MMP were assayed from 333 colorectal cancer using polymerase chain reaction-restriction fragment length polymorphism.

RESULTS

TIMP2-418G/G, TIMP2 303G/G and MMP9-1562C/C were more frequent in patients than in controls (P = 0.020, P < 0.0001 and P < 0.044, respectively). Frequency of TIMP2-418G/G was higher in patients with metastasis than in those without metastasis, and that of TIMP2 303G/G was higher in patients with rectal cancer than in those with colon cancer (P = 0.008 and P =0.022, respectively). TIMP2-303A/A and MMP2-1575G/G were less frequent in patients than in controls (P = 0.001 and P = 0.005, respectively). The TIMP2-418G303G haplotype was more frequent (P < 0.0001) and MMP2-1575G-735C haplotype was less frequent in patients than in controls (P= 0.005).

CONCLUSION

Specific single-nucleotide polymorphism in TIMP2 and MMP appeared to be associated with tumorigenesis and biological behavior in colorectal cancer, which is expected be further verified in a larger cohort in the future.